Study on establishment of high precise determination method for nondigestible oligosaccharide

2014 Fiscal Year Final Research Report

• Project/Area Number: 24650498
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Nagasaki
• Principal Investigator: TANABE Kenichi 長崎県立大学, 看護栄養学部, 助教 (60585727)
• Co-Investigator(Kenkyū-buntansha): OKU Tsuneyuki 十文字学園女子大学, 人間生活学部, 客員教授 (50010096) ; NAKAMURA Sadako 十文字学園女子大学, 食・栄養・健康研究所, 客員研究員 (60382438)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 難消化性オリゴ糖 / 定量法 / 食品分析 / プレバイオティクス / 難消化性糖質 / 食物繊維 / ルミナコイド

Outline of Final Research Achievements

Developing an accurate quantitative method for nondigestible oligosaccharides is a long-term project. Association of Official Analytical Chemists (AOAC) method 2009.01 was recently developed. However, the method may not be able to measure nondigestible oligosaccharides accurately. The objectives of this study were to clarify the inaccuracy of AOAC method 2009.01 for the measurement of nondigestible oligosaccharides and to propose an improved method.
AOAC method 2009.01 was able to hydrolyze digestible oligosaccharides. The method could not accurately quantify oligosaccharides because it could not be used to distinguish between digestible and nondigestible oligosaccharides. To solve this problem, we proposed an improved method using porcine intestinal enzymes instead of amyloglucosidase. The improved method completely hydrolyzed digestible oligosaccharides, and distinguished nondigestible oligosaccharide from digestible oligosaccharide in commercially available processed food.

Free Research Field

保健栄養学