2013 Fiscal Year Final Research Report
Detection of cancer specific T cells on a chip
| Project/Area Number |
24650630
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor immunology
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| Research Institution | University of Toyama |
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Principal Investigator |
KISHI HIROYUKI 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210)
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| Co-Investigator(Kenkyū-buntansha) |
MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
OZAWA Tatsuhiko 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105)
KOBAYASHI Eiji 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Shuichi 金沢大学, 大学院医薬保健学総合研究科, 教授 (60185923)
MIZUKOSHI Eishiro 金沢大学, 医学部附属病院, 講師 (90345611)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞免疫 / 細胞チップ |
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| Research Abstract |
Cancer-specific cytotoxic T lymphocytes play important roles in cancer immunity. Previously we developed microwell-array chips that have an array of about 250,000 microwells in which only single lymphocytes can be accommodated. In this study, we showed that we could detect antigen-specific T lymphocytes by culturing single lymphocytes in each microwell, stimulating them with antigenic peptides, and detecting their cytokine-secretion on the chip. By retrieving the detected single T lymphocytes, we could amplify TCRalpha/beta cDNAs, insert them in a retrovirus vector with homologous recombination, and examine their antigen-specificity by expressing them on a TCR-deficient T cell line. The whole process can be accomplished within 10 days. The system may greatly contribute to the cancer immunotherapy in the future.
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