2013 Fiscal Year Final Research Report
Identification of specific cancer stem cell markers for diagnosis
Project/Area Number |
24650632
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Tumor diagnosis
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Research Institution | The University of Tokyo |
Principal Investigator |
IMAI Kohzoh 東京大学, 医科学研究所, 特任教授 (60117603)
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Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Hiroaki 東京大学, 医科学研究所, 特任准教授 (90563289)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Keywords | 腫瘍マーカー / がん幹細胞 / がん / 遺伝子 / 臨床 |
Research Abstract |
We started our project to identfy specific cancer stem cell markers for diagnosis. ES cell markers and phenotypic markers of cancer proliferation are used to identify CSCs. We already have reported that PRDM14 expression is enhanced in clinical BC cases. We also show that KLF2 repression has an important role in EZH2 oncogenesis in breast and prostate cancers. PRDM14 and KLF2 are known to be important for ES cells maintainance. Accoding to our planning, we collected sphere-forming cells or SP fractions using a cell sorter in order to obtain cancer cells with stemness phenotypes from clinical cancer tissues. After that, we applied samples from those cells and normal control cells for a next generation sequencer to obtain the candidate genes. We could obtain several candidate genes for specific cancer stem cell markers and already have finished functional analysis for them. Now, we proceed to analyse clinical breast and pancreatic cancer tissues for specific cancer stem cell markers.
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Research Products
(19 results)
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[Journal Article] Mesenchymal stem cells cancel azoxymethane-induced tumor initiation2014
Author(s)
Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y
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Journal Title
STEM CELLS
Volume: 32(4)
Pages: 913-925
DOI
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[Journal Article] The effect of IGF-I receptor blockade for human esophageal squamous cell carcinoma and adenocarcinoma2014
Author(s)
Adachi Y, Ohashi H, Imsumran A, Yamamoto H, Matsunaga Y, Taniguchi H, Nosho K, Suzuki H, Sasaki Y, Arimura Y, Carbone DP, Imai K, Shinomura Y
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Journal Title
Tumor Biol
Volume: 35(2)
Pages: 973-985
DOI
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[Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk2013
Author(s)
Suzuki R, Yamamoto E, Nojima M, Maruyama R, Yamano HO, Yoshikawa K, Kimura T, Harada T, Ashida M, Niinuma T, Sato A, Nosho K, Yamamoto H, Kai M, Sugai T, Imai K, Suzuki H, Shinomura Y
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Journal Title
J Gastroenterol
Volume: (Epub ahead of print)
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[Journal Article] Methylation of a panel of microRNA genes is a novel biomarker for detection of bladder cancer2013
Author(s)
Shimizu T, Suzuki H, Nojima M, Kitamura H, Yamamoto E, Maruyama R, Ashida M, Hatahira T, Kai M, Masumori N, Tokino T, Imai K, Tsukamoto T, Toyota M
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Journal Title
Eur Urol
Volume: 63(6)
Pages: 1091-1100
DOI
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[Journal Article] Association between genomic alterations and metastatic behavior of colorectal cancer identified by array-based comparative genomic hybridization2013
Author(s)
Sawada T, Yamamoto E, Suzuki H, Nojima M, Maruyama R, Shioi Y, Akasaka R, Kamimae S, Harada T, Ashida M, Kai M, Adachi Y, Yamamoto H, Imai K, Toyota M, Itoh F, Sugai T
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Journal Title
Genes Chromosomes Cancer
Volume: 52(2)
Pages: 140-149
DOI
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[Journal Article] Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids2013
Author(s)
Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y
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Journal Title
World J Gastroenterol
Volume: 19(11)
Pages: 1718–1727
DOI
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[Journal Article] Conditioned mesenchymal stem cells produce pleiotropic gut trophic factors2013
Author(s)
Watanabe S, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nasuno M, Yamashita K, Idogawa M, Naishiro Y, Murata M, Adachi Y, Fujimiya M, Imai K, Shinomura Y
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Journal Title
J Gastroenterol
Volume: 49(2)
Pages: 270-282
DOI
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[Journal Article] Characteristics of Japanese inflammatory bowel disease susceptibility loci2013
Author(s)
Arimura Y, Isshiki H, Onodera K, Nagaishi K, Yamashita K, Sonoda T, Matsumoto T, Takahashi A, Takazoe M, Yamazaki K, Kubo M, Fujimiya M, Imai K, Shinomura Y
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Journal Title
J Gastroenterol Aug 13
Volume: Epub ahead of print
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[Journal Article] Molecular dissection of premalignant colorectal lesions reveals early onset of the CpG island methylator phenotype2012
Author(s)
Yamamoto E, Suzuki H, Yamano H, Maruyama R, Nojima M, Kamimae S, Sawada T, Ashida M, Yoshikawa K, Kimura T, Harada T, Suzuki R, Sato A, Kai M, Sasaki Y, Tokino T, Sugai T, Imai K, Shinomura Y, Toyota M
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Journal Title
Am J Pathol
Volume: 18
Pages: 1847-1861
DOI
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[Journal Article] Aberrant methylation of RASGRF1 is associated with an epigenetic field defect and increased risk of gastric cancer2012
Author(s)
Takamaru H, Yamamoto E, Suzuki H, Nojima M, Maruyama R, Yamano H, Yoshikawa K, Kimura T, Harada T, Ashida M, Suzuki R, Yamamoto H, Kai M, Tokino T, Sugai T, Imai K, Toyota M, Shinomura Y
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Journal Title
Cancer Prev Res
Volume: 5
Pages: 1203-1212
DOI
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[Journal Article] Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors2012
Author(s)
Niinuma T, Suzuki H, Nojima M, Nosho K, Yamamoto H, Takamaru H, Yamamoto E, Maruyama R, Nobuoka T, Miyazaki Y, Nishida T, Bamba T, Kanda T, Ajioka Y, Taguchi T, Okahara S, Takahashi H, Nishida Y, Hosokawa M, Hasegawa T, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y
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Journal Title
Cancer Res
Volume: 72(5)
Pages: 1126-1136
DOI
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[Journal Article] Silencing of the Kruppel-like factor 2 by the histone methyltransferase EZH2 in human cancer2012
Author(s)
Taniguchi H, Jacinto F.V., Villanueva A, Fernández AF, Yamamoto H, Carmona F.J, Puertas S, Marquez VE, Shinomura Y, Imai K, and Esteller M
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Journal Title
Oncogene
Volume: 31
Pages: 1988-1994
DOI
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[Journal Article] A DNA methylation fingerprint of 1628 human samples2011
Author(s)
Fernandez AF, Assenov Y, Martin-Subero JI, Balint B, Siebert R, Taniguchi H, Yamamoto H, Hidalgo M, Tan AC, Galm O, Ferrer I, Sanchez-Cespedes M, Villanueva A, Carmona J, Sanchez-Mut JV, Berdasco M, Moreno V, Capella G, Monk D, Ballestar E, Ropero S, Martinez R, Sanchez-Carbayo M, Prosper F, Agirre X, Fraga MF, Graña O, Perez-Jurado L, Mora J, Puig S, Prat J, Badimon L, Puca AA, Meltzer SJ, Lengauer T, Bridgewater J, Bock C, Esteller M
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Journal Title
Genome Res
Volume: 22(2)
Pages: 407-419
DOI
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