2012 Fiscal Year Final Research Report
Generation of monoclonal antibodies targeting induced cancer stem cells
| Project/Area Number |
24650636
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Yokohama City University |
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Principal Investigator |
RYO Akihide 横浜市立大学, 医学研究科, 教授 (20363814)
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| Project Period (FY) |
2012
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| Keywords | 細胞 / 再生医学 / 癌 / トランスレーショナルリサーチ / 抗体 |
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| Research Abstract |
To obtain monoclonal antibodies specifically targeting cancer stem cells, we immunized rat with induced cancer stem cells (iCSC) followed by the production of hybridomas. By using iCSC and breast cancer cell lines, we selected two monoclon al colones that only recognize iCSC but not brest cancer cell line. Protein array analysis revealed that ABL1 could be a target of the monoclonal antibodies. When iCSCs were treated with an ABL inhibitor, imatinib, self-renewal property of iCSCs was inhibited.
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[Journal Article] Induction of cells with cancer stem-cell properties from non-tumorigenic human mammary epithelial cells by defined reprogramming factors2013
Author(s)
Nishi M, Sakai Y, Akutsu H, Nagashima Y, Quinn G, Masui S, Kimura H, Perrem K, Umezawa A, Yamamoto N, Lee SW, Ryo A
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Journal Title
DOI
Peer Reviewed
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