2013 Fiscal Year Final Research Report
Isolation of tumor-derived exosomes using NHq method and their appreciation for exploration of biomarker
| Project/Area Number |
24650637
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | Osaka City University |
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Principal Investigator |
SHIOTA Masayuki 大阪市立大学, 医学(系)研究科(研究院), 講師 (30381990)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 熱ショックタンパク質 / エキソソーム |
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| Research Abstract |
Recent research has reported that HSP72 released from tumor cells on surface of exosome. Therefore, we aimed to assess the NHq method, which we established method to isolate of HSP72-binding proteins, for purification of exosome and to explore for biomarker. HSP72-binding molecules isolated with NHq could not involve CD63 and CD9. These markers of exosome were detected in flow-through of NHq method. HSP72 was mainly detected in the supernatant of ultracentrifugation. HSP72 did not exist on surface of exosome against previous report. Therefore, we concluded that NHq method could not isolate exosome. Whereas, we tried to explore the biomarker of multiple myeloma using NHq method. As a result, we identified 3 proteins in sera of patients. One of these proteins might be a biomarker of multiple myeloma.
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[Journal Article] Lipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells2014
Author(s)
Sano S, Izumi Y, Yamaguchi T, Yamazaki T, Tanaka M, Shiota M, Osada-Oka M, Nakamura Y, Wei M, Wanibuchi H, Iwao H, Yoshiyama M
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Journal Title
Biochem Biophys Res Commun
Volume: 445
Pages: 327-333
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[Journal Article] Hsc70 Contributes to Cancer Cell Survival by Preventing Rab1A Degradation under Stress Conditions
Author(s)
Tanaka M, Mun S, Harada A, Ohkawa Y, Inagaki A, Sano S, Takahashi K, Izumi Y, Osada-Oka M, Wanibuchi H, Yamagata M, Yukimura T, Miura K, Shiota M, Iwao H
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Journal Title
PLOS ONE
Volume: (in press)
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