2012 Fiscal Year Final Research Report
Proteomic analysis of tumor-derived nanovesicles and its application for innovative diagnostic method.
| Project/Area Number |
24650641
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
TSUNODA Shin-ichi 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (90357533)
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| Project Period (FY) |
2012
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| Keywords | 医療・福祉 / 癌 / 生体分子 / プロテオーム |
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| Research Abstract |
This is a challenging study to explore biomarkers on tumor cell-derived nanovesicles (exosomes) and to investigate their potential as innovative diagnostic markers for early detection and molecular characterization of cancer, and for decision making in cancer treatment. Here, we performed a focused proteome analysis for the proteins of exosomes, which are recently revealed to be membrane vesicles secreted from various cells including tumor cells. At first, tumor cell-derived exosomes were collected by ultracentrifugation method from cell culture supernatant. The finally collected products were confirmed to be purified exosomes by morphology of electron microscopy, particle size by dynamic light scattering and expression of tetraspanins, which were markers of exosomes, by western blotting. Then, proteins were extracted from the purified exosomes and analyzed the expressed proteins by mass spectrometry. These experiments revealed that various tumor-related membrane proteins were detectable in tumor-derived exosomes. In the future, we will test the feasibility of exoxome-based in vitro diagnosis by isolating the tumor-derived exoxomes from the peripheral blood of patients and profiling tumor-related proteins of them.
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[Journal Article] Proteomic analysis of the hippocampus in Alzheimer's disease model mice by using two-dimensional fluorescence difference in gel electrophoresis2013
Author(s)
Takano M., Yamashita T., Nagano K., Otani M., Maekura K., Kamada H., Tsunoda S., Tsutsumi Y., Tomiyama T., Mori H., Matsuura K., Matsuyama S
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Journal Title
Neuroscience Letters
Volume: 534
Pages: 85-9
DOI
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[Journal Article] Annexin A4 is a possible biomarker for cisplatin susceptibility of malignant mesothelioma cells2012
Author(s)
Yamashita T., Nagano K., Kanasaki S., Maeda Y., Furuya T., Inoue M., Nabeshi H., Yoshikawa T., Yoshioka Y., Itoh N., Abe Y., Kamada H., Tsutsumi Y., Tsunoda S
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 421(1)
Pages: 140-4
DOI
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