2013 Fiscal Year Final Research Report
Non-invasive monitoring of environmental stress on plant using functional optical coherence tomography
| Project/Area Number |
24651023
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YONEKURA Tetsushi 埼玉県環境科学国際センター, 自然環境担当, 研究員 (40425658)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 光断層画像法 / オゾンストレス / バイオスペックル / 植物活性 / 環境ストレス / 環境汚染評価 / 非破壊検査 |
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| Research Abstract |
In this study, a novel and non-invasive monitoring method of biological activities of plant under environmental stress was developed using an optical coherence tomography (OCT) together with a statistical properties of biospeckle. The biospeckle is generated by the dynamic motion of organelles inside a cell, and hence can reflect the biological activities of plant leaf. The proposed functional OCT system experimentally demonstrated to observe three-dimensionally the biological activities of plant under ozone stress. To analyze the biospeckle signal, two types of analysis were proposed and examined. One is based on the magnitude of the fluctuation (standard deviation) of the signal. The other one is to analyze temporal characteristics of the signal, i.e., a autocorrelaton function of the signal. As experimental results of ozone exposure, it was revealed that those two methods could immediately detect an independent response about environmental condition.
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