Mechanism of the spinal deformity of fish born in heavy oil polluted seawater, and rescue with the new detoxification protein

2013 Fiscal Year Final Research Report

• Project/Area Number: 24651044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kanazawa University
• Principal Investigator: HAYAKAWA Kazuichi 金沢大学, 薬学系, 教授 (40115267)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Nobuo 金沢大学, 環日本海域環境研究センター, 准教授 (60242476) ; OSHIMA Yuji 九州大学, 大学院農学研究院, 教授 (70176874)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 生物影響 / 骨代謝 / 胚発生 / 解毒タンパク質

Research Abstract

Using bioassay systems with fishes, we examined the mechanism of spinal deformity of fish by the heavy oil pollution. As a result, hydroxylated polycyclic aromatic hydrocarbons (OHPAH) provided causing death in early embryogenesis of the medaka, and OHPAH influenced the cell activities in fish scales. We suggested that the main substances of poison causing deformity of the fish are OHPAHs. Furthermore, we found the material which could become a candidate of the detoxification protein.

Research Products

• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish (2014)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., Toriba, A., Hayakawa, K., Chowdhury, V.S., Endo, M., Chiba, A., Sekiguchi, T., Nakano, M., Tabuchi, Y., Kondo, T., Wada, S., Mishima, H., Hattori, A., Suzuki, N.
  • Journal Title: Env. Sci. Poll. Res. Volume: 21 (10) Pages: 6365-6372
  • DOI: 10.1007/s11356-012-1347-5
  • Peer Reviewed
• [Journal Article] Increased Prostaglandin E_2 has a positive correlation with plasma calcium during goldfish reproduction (2014)
  • Author(s): Suzuki, N., Maruyama, Y., Nakano, M., Hattori, A., Honda, M., Shimasaki, Y., Sekiguchi, T., Hishima, H., Wada, S., Srivastav, A.K., Hayakawa, K., Oshima, Y.
  • Journal Title: J. Fac. Agr., Kyushu Univ. Volume: 59 (1) Pages: 97-101
  • URL: http://www.agr.kyushu-u.ac.jp/english/journal/
  • Peer Reviewed
• [Journal Article] のと海洋ふれあいセンターが観測した沿岸水のpH低下について (2014)
  • Author(s): 鈴木元気、牧野史弥、早川和一、東出幸真、達克幸、坂井恵一
  • Journal Title: のと海洋ふれあいセンター研究報告 Volume: 19 Pages: 1-6
  • URL: http://notomarine.jp/rep-list.htm
  • Peer Reviewed
• [Journal Article] Zebrafish scales respond differently to in vitro dynamic and static acceleration : Analysis of interaction between osteoblasts and osteoclasts (2013)
  • Author(s): Kitamura, K., Tanahira, K., Inari, M., Satoh, Y., Hayakawa, K., Tabuchi, Y., Ogai, K., Nishiuchi, T., Kondo, T., Mikuni-Takagaki, Y., Chen, W., Hattori, A., Suzuki, N.
  • Journal Title: Comp. Biochem. Physiol., Part A Volume: 166 Pages: 74-80
  • DOI: 10.1016/j.cbpa.2013.04.023
  • Peer Reviewed
• [Journal Article] Biological Effects of Polycyclic Aromatic Hydrocarbon Derivatives (2013)
  • Author(s): Bekki, K., Toriba, A., Tang, N., Kameda, T., Hayakawa, K.
  • Journal Title: J. UOEH Volume: 35 (1) Pages: 17-24
  • DOI: 10.7888/juoeh.35.17
  • Peer Reviewed
• [Journal Article] Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales (2013)
  • Author(s): Yano, S., Kitamura, K., Satoh, Y., Nakano, M., Hattori, A., Sekiguchi, T., Ikegame, M., Nakashima, H., Omori, K., Hayakawa, K., Chiba, A., Sasayama, Y., Ejiri, S., Mikuni-Takagaki, Y., Mishima, H., Funahashi, H., Sakamoto, T., Suzuki, N.
  • Journal Title: Zoological Sci. Volume: 30 (3) Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Determination of calcium sensing receptor in the scales of goldfish and induction of its mRNA expression by acceleration loading (2012)
  • Author(s): Kakikawa, M., Yamamoto, T., Chowdhury, V. S., Satoh, Y., Kitamura, K., Sekiguchi, T., Funahashi, H., Omori, K., Endo, M., Yano, S., Yamada, S., Hayakawa, K., Chiba, A., Srivastav, A. K., Ijiri, K., Seki, A., Hattori, A., Suzuki, N.
  • Journal Title: Biol. Sci. Space Volume: 26 Pages: 26-31
  • DOI: 10.2187/bss.26.26
  • Peer Reviewed
• [Journal Article] 魚類のウロコを用いた評価系の開発と骨代謝研究への応用 (2012)
  • Author(s): 鈴木信雄、舟橋久幸、耿啓達、柿川真紀子、山田外史、廣田憲之、北村敬一郎、清水宣明、早川和一、三島弘幸、岩坂正和、上野照剛、大森克徳、矢野幸子、池亀美華、田渕圭章、和田重人、近藤隆、服部淳彦
  • Journal Title: まぐね/Magune Jpn. Volume: 7 (4) Pages: 174-178
  • URL: http://www.magnetics.jp/journal/mag_2012/0704.html
  • Peer Reviewed
• [Journal Article] Prostaglandin E_2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish (2012)
  • Author(s): Omori, K., Wada, S., Maruyama, Y., Hattori, A., Kitamura, K., Sato, S., Nara, M., Funahashi, H., Yachiguchi, K., Hayakawa, K., Endo, M., Kusakari, R., Yano, S., Srivastav, A. K., Kusui, T., Ejiri, S., Chen, W., Tabuchi, Y., Furusawa, Y., Kondo, T., Sasayama, Y., Nishiuchi, T., Nakano, M., Sakamoto, T., Suzuki, N.
  • Journal Title: Zoological Sci. Volume: 29 (8) Pages: 499-504
  • DOI: 10.2108/zsj.29.499
  • Peer Reviewed
• [Journal Article] Analysis of 8-hydroxy-2'-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry (2012)
  • Author(s): Hosozumi, C., Toriba, A., Chuesaard, T., Kameda, T., Tang, N., Hayakawa, K.
  • Journal Title: J. Chromatography B Volume: 893-894 Pages: 173-176
  • DOI: 10.1016/j.jchromb.2012.02.043
  • Peer Reviewed
• [Journal Article] Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei : Possible physiological roles of the calcitonin family in osmoregulation (2012)
  • Author(s): Suzuki, N., Sekiguchi, T., Satake, H., Kato, K., Nishiyama, Y., Takahashi, H., Danks, J.A., Martin, T.J., Hattori, A., Nakano, M., Kakikawa, M., Yamada, S., Ogoshi, M., Hyodo, S., Yamaguchi, Y., Chowdhury, V.S., Hayakawa, K., Funahashi, H., Sakamoto, T., Sasayama, Y.
  • Journal Title: Gene Volume: 499 Pages: 326-331
  • DOI: 10.1016/j.gene.2012.03.042
  • Peer Reviewed
• [Presentation] 能登半島における過去8年間の多環芳香族炭化水素類及び粒子状物質の大気内変動 (2014)
  • Author(s): 袴田真理子、唐寧、亀田貴之、鳥羽陽、早川和一
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本大学
  • Year and Date: 20140327-30
• [Presentation] 立山室堂の積雪層の陰イオンと多環芳香族炭化水素類の分析 (2013)
  • Author(s): 袴田真理子、鈴木元気、牧野史弥、立松路也、唐寧、鳥羽陽、青木一真、早川和一
  • Organizer: 第30回イオンクロマトグラフィー討論会
  • Place of Presentation: 株式会社豊田中央研究所
  • Year and Date: 20131128-29
• [Presentation] 多環芳香族炭化水素類の魚類の骨代謝に対する作用 (2013)
  • Author(s): 表俊樹、川部季美、北村敬一郎、服部淳彦、田淵圭章、近藤隆、鳥羽陽、早川和一、鈴木信雄
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ
  • Year and Date: 20131024-26
• [Presentation] ポリ塩化ビフェニル(PCB118)は魚の骨代謝を攪乱する (2013)
  • Author(s): 谷内口孝治、関口俊男、羽賀雄紀、松村千里、鶴川正寛、中野武、北村敬一郎、鳥羽陽、早川和一、近藤隆、田渕圭章、和田重人、遠藤雅人、服部淳彦、鈴木信雄
  • Organizer: 第84回日本動物学会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130926-28
• [Presentation] Determination of nitropolycyclic aromatic hydrocarbons in water samples (2013)
  • Author(s): Chondo, Y., Nassar, H. F., Yoshida, Y., Li, Y., Kameda, T., Toriba, A., Hayakawa, K.
  • Organizer: China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: Fukuoka, Kyushu University
  • Year and Date: 20130822-24
• [Presentation] Atmospheric polycyclic aromatic hydrocarbons in Noto Peninshula (2013)
  • Author(s): Hakamata, M.
  • Organizer: Japan from 2004. China-Japan-Korea Symposium on Analytical Chemistry, 2013
  • Place of Presentation: Fukuoka, Kyushu University
  • Year and Date: 20130822-24
• [Presentation] Development and application of a fish scale in vitro assay system (2013)
  • Author(s): Suzuki, N.
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe convention center & Kobe portopia hotel
  • Year and Date: 20130528-0601
• [Presentation] キンギョの再生ウロコにおける隆起線形成リズム (2012)
  • Author(s): 黒田美翔、舟橋久幸、鬼木弘明、宇都理佳、筒井和義、鈴木信雄、服部淳彦
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井
  • Year and Date: 20121130-1201
• [Presentation] Polychlorinated biphenyl disrupts bone metabolism in goldfish (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., Toriba, A., Hayakawa, K., Hattori, A., Suzuki, N.
  • Organizer: 7th International PCB Workshop
  • Place of Presentation: Palais des CongrÈs of Arcachon
  • Year and Date: 20120527-31