2013 Fiscal Year Final Research Report
The construction of a module to visualize DNA damage signaling in live cells
| Project/Area Number |
24651046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
FURUYA Kanji 京都大学, 放射線生物研究センター, 講師 (90455204)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 蛍光タンパク質 / チェックポイント / 細胞周期 / ライブイメージング |
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| Research Abstract |
DNA checkpoint mechanism is activated when cells receive the DNA damage stress that arose from gamma-ray exposure. Not only the DNA damage responsive signaling cascade, DNA damage checkpoint could act as anti-tumour mechanism to protect us from cancer. Thus to detect which cells activate DNA checkpoint could help detect precancerous cells. Here we tackled to construct the module to detect checkpoint activation in live cells. We constructed the GFP-tagged peptide to express in the cells, and the module translocate outside the nucleus upon DNA replicative stress. Now we are modifying the module to make it more clear response so that we could apply to monitor checkpoint activated cells in live organism.
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