2013 Fiscal Year Final Research Report
The development of a new technique to add DNA methylation on the target DNA sequence for the realization of epigenetic toxicity analysis of validation type.
| Project/Area Number |
24651065
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | トキシコロジー / エピジェネティック毒性 |
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| Research Abstract |
To advance the research for "epigenetic toxicity", which is thought to be the mechanism for the delayed toxic effects of chemicals, a new technique for editing DNA methylation on the target DNA sequence was tried to develop in this study. For that, "DNA binding protein-nuclease" techniques which are in the stage of practical use, was applied. In addition, an original idea to achieve the specificity comparable to PCR technique was also applied. Though preliminary, data indicating the possibility for the successful development of the technique was obtained.
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