2013 Fiscal Year Final Research Report
Analysis of metabolic network in dechlorinating enrichment culture and construction of model system
| Project/Area Number |
24651075
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Shizuoka University |
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Principal Investigator |
FUTAMATA Hiroyuki 静岡大学, 工学(系)研究科(研究院), 教授 (50335105)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 環境浄化 / トリクロロエテン / バイオレメディエーション / 脱塩素化 / Dehalococcoides / Methanogen / 競合 |
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| Research Abstract |
The mechanism of hydrogen interspecies transfer which enables to dechlorinate trichloroethene (TCE) effectively was characterized. Methane production was observed during TCE-dechlorination in the non-effective dechlorinating culture, while methane was produced rapidly after TCE-dechlorination in the effective dechlorinating culture. Molecular techniques revealed that aceticlastic methanogens competed with Dechlorinator, on the other hand, hydrogenotrophic methanogens did not. These results suggested that the activity of anaerobic acetate oxidizing bacteria play a key role for efficient dechlorination. We succeeded to isolate anaerobic acetate oxidizing bacteria, closely related to Moraxella osloensis.
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