Studies of molecular mechanism in phytoremediation of plants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24651087
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: Fukuyama University
• Principal Investigator: OHTA Masaya 福山大学, 生命工学部, 教授 (00203802)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 環境修復技術 / フィトレメディエーション

Outline of Final Research Achievements

To clarify the molecular mechanisms of uptake and high accumulation to PCB residues by Solidago canadenisis and Phytolacca americana L., it was attempted to use glycolipids obtained from these plants for assays of PCB congeners in the transgenic Arabidopsis plants carrying a reconbinant guinea pig AhR-mediated GUS reporter gene expression system. The glycolipids SG from the roots and DGDG from the leaves of S. canadenisis, and triterpenoidal glycosides (phytolalaccasapnins) from the leaves of P. americana L. increased PCB126-induced GUS activity. In contrast, SG from soy beads and DGDG from wheat did not affect PCB126-induced GUS activity. These results suggested glycolipids SG of S. canadenisis appeared to increase the uptake of PCB126, and glycolipids DGDG appeared to transport PCB126 into aerial parts. Moreover the present study suggested that possible to increase the sensitivity of the monitoring by adding these glycolipids.

Free Research Field

糖鎖生物学