Rapid Caputure System of Circulationg Tumor Cells by use of Microfiber

2014 Fiscal Year Final Research Report

• Project/Area Number: 24651158
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: The University of Tokyo
• Principal Investigator: MADOKA Takai 東京大学, 工学(系)研究科(研究院), 教授 (40287975)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マイクロファイバー / 血中循環がん細胞 / イムノアッセイ

Outline of Final Research Achievements

Three-dimensional polystyrene (PS) microfiber fabric with vacuum system was developed for capturing circulating tumor cells (CTCs) within a short time. Various microfiber fabrics with different diameters were prepared by the electrospinning method and optimized for contact frequency of cells. The vacuum filtration utilizing these microfiber fabrics could filter cells without mechanical damage. The fabric system with immobilized anti-EpCAM antibodies was able to specifically capture MCF-7 cells that express EpCAM on their cell fabric within seconds. Also, the specificity of the system was tested by monitoring the ability to isolate MCF-7 cells from a mixture with CCRF-CEM cells that do not express EpCAM. Furthermore, the capture ability of the microfiber remained intact even when tested with whole blood of pigs spiked with MCF-7 cells.

Free Research Field

ナノ・マイクロ科学