2013 Fiscal Year Final Research Report
chromosome conformation capture variant technique for disease risk genomic loci
| Project/Area Number |
24651221
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical genome science
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| Research Institution | Niigata University |
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Principal Investigator |
HASEGAWA Mai 新潟大学, 脳研究所, 研究員 (50596153)
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| Co-Investigator(Renkei-kenkyūsha) |
KUWANO Ryozo 新潟大学, 脳研究所, 教授 (20111734)
NAKAYA Akihiro 新潟大学, 超域学術院, 准教授 (60301149)
MIYASHITA Akinori 新潟大学, 脳研究所, 助教 (60323995)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 核内高次構造 / クロマチン構造 / 発現制御 / ポストGWAS |
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| Research Abstract |
Recent case-control genome-wide association studies (GWAS) have identified numerous single nucleotide polymorphisms (SNPs) which act as genetic risk factor, but most of SNPs which located on non-coding region are still unknown for their function. Since such SNP is predicted to act as a distal enhancer of responsible gene, we have developed a chromosome conformation capture method suitable for post-GWAS analysis. We demonstrated that tethered conformation capture (a comprehensive chromatin conformation capture method) and following pulldown using biotinylated RNA probe which is targeted to risk SNP region can detect spatially proximate genomic loci efficiently.
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Research Products
(5 results)
