Preparation of the stem cells from homofused spermatids by monitoring Cadm1 marker

2015 Fiscal Year Final Research Report

• Project/Area Number: 24651223
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Tokyo Medical University (2015); International University of Health and Welfare (2012-2014)
• Principal Investigator: MOMOI TAKASHI 東京医科大学, 医学部, 客員教授 (40143507)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: spermatogonia / Cadm1 / stem cells / integrin / survival signal

Outline of Final Research Achievements

It is possible to establish the pluripotent germinal stem cells by incubating the spermatogonia cells in the medium containing GDNF, LIFm FGF and FGF etc. To overcome the ethical problems to the human germ cells, we tried to prepare the germinal stem cells by the method of electronic fusion of spermatids. We prepared the Tg mouse expressing Cadm1 promoter-fluorescent proteins, which specifically express them in the spermatogonia. We isolated spermatids from testis of Tg mouse, fused them and incubated for about 1 to 2 month. However, they could not permanently live under the condition because of the lack of integrin survival signals, RA70/Scap-2, during isolation of spermatids from testis.

Free Research Field

細胞分子生物学