2012 Fiscal Year Final Research Report
Capturing of identical chromosome
| Project/Area Number |
24651224
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TARUI Hiroshi 独立行政法人理化学研究所, LSAシステム構築ユニット, 上級研究員 (90342815)
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| Project Period (FY) |
2012
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| Keywords | ゲノム / クロモソーム / 選別 |
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| Research Abstract |
This project is aimed to develop a method for collecting identical chromosome with high purity. By weak sonication, intact and loosed chromosome could be obtained from cells arrested on metaphase. Next, chromosome isolation strategy was designed and constructed for targeted 9^<th> chromosome. At the same time, quality control strategy based on qPCR was also prepared. The term planned has been passed, however the experiment is continuously performed.
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