Genome-wide in vivo footprinting by next-generation sequencing

2013 Fiscal Year Final Research Report

• Project/Area Number: 24651226
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Takashi 東京大学, 理学(系)研究科(研究院), 教授 (90201326)
• Project Period (FY): 2012-04-01 – 2013-03-31
• Keywords: ゲノム解析技術

Research Abstract

Proteins that act on genomic DNA functionally regulate the genome. Accordingly, if we can comprehensively reveal the interactions between genomic DNA and proteins in living cells, we will substantially deepen our understanding of how the genome works. As a novel method toward this goal, we tried to develop a method termed DMS-Seq that uses dimethylsulfate (DMS) and next-generation sequencing. Following the optimization of the method, we applied it to the baker's yeast as a model system and successfully detected signals on regions known to regulate gene expression. These results would suggest the feasibility of this approach.