2013 Fiscal Year Final Research Report
Alteration of the level of Notch signaling activity in mouse embryo by gene duplication of Notch
| Project/Area Number |
24651230
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
BESSHO Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70261253)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKUMURA Yuich 愛知県立大学, 情報科学部, 准教授 (50324968)
MATSUI Takaaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60403333)
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| Research Collaborator |
NAKAHATA Yasukazu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50390810)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Notchシグナリング / ノックインマウス / 体節形成 |
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| Research Abstract |
Manipulation of intracellular signaling activity in embryos is an effective tool for analysis of dynamic phenomena in developing embryo. However, it is very difficult to alter the intracellular signaling activity stably and quantitatively. We tried to generate knockin mice in which Notch1 gene is duplicated, thereby increasing the number of Notch1 allele. By using this knockin mice, we can generate mouse embryos with various number of Notch1 allele from 0 to 4, thus we can stably and quantitatively alter the Notch signaling activity in this knockin mouse line. With this Knockin mouse the analyses of Notch signal activity in dynamic phenomena in embryo such as somitogenesis are available.
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[Journal Article] 3'UTR-dependent regulation of mRNA turnover is critical for differential distribution patterns of cyclic gene mRNAs2014
Author(s)
Nitanda, Y., Matsui, T., Matta, T., Higami, A., Kohno, K., Nakahata, Y., and Bessho, Y
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Journal Title
FEBS Journal
Volume: 281
Pages: 146-156
DOI
Peer Reviewed
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