2014 Fiscal Year Final Research Report
The development of micro-imaging method to realize simultaneous detection of metabolism and localization of microbe
| Project/Area Number |
24651232
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied genomics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 応用微生物 / がん / アセトアルデヒド / 腸内細菌 |
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| Outline of Final Research Achievements |
Habitual high amount of drinking induce cancer of human large intestine. The reason of drinking-related cancer is considered as an effect of acetaldehyde, which is produced by enterobacterium as a metabolite of ethanol. Object of this study is to confirm a production of acetaldehyde by enterobacterium on the surface of mucous membrane of human large intestine, and to visualize a production of acetaldehyde from bacterial cells.
First, I confirmed the amount of acetaldehyde production by colon enterobacterium isolated from feces of alcoholics. As a result, specific genus, which were not discussed with colon cancer, were concerned to play an important role in acetaldehyde production. Subsequently, cells of objective genus were visualized by fluorescence in situ hybridization. Localized cells of specific stained microbe were observed on the surface of biopsy.
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| Free Research Field |
微生物生態学
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