2013 Fiscal Year Final Research Report
Chalenge to make "genome swapping" using Streptomyces linear genome.
| Project/Area Number |
24651233
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IKEDA Haruo 北里大学, 北里研究所, 教授 (90159632)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ゲノムスワップ / 放線菌 / 枯草菌 / ツインゲノム |
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| Research Abstract |
Toward establishment of bacteria having artificial twin genome functions, we tried to make Steptomyces lividans having whole Bacillus subtilis genome on the linear chromosome. We established cytoplasmic mixing method by using protoplast fusion between S. lividans and B. subtilis. To make twin genome functions, we applied site-specific integration system from actinophage phiC 31. In the case of using plasmid as the guest genome, we succeed to integrate into the host genome as on the experimental design. In the case of whole B. subtilis genome, we could detect the integration phenomena as antibiotic resistance, but we were not able to detect B. subtilis genome on the host genome. This impossibility may be caused from large GC content difference of both genomes. To take another approach to make the twin genome, we try to make conjugative transfer of large DNA from Eschericia coli to B. subtilis, and we make stable and novel protocol for the inter-species conjugative transfer.
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