Autoregulator addition strategy to awakening cryptic biosynthetic genes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24651243
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: NIHIRA Takuya 大阪大学, 生物工学国際交流センター, 教授 (70144441)
• Co-Investigator(Kenkyū-buntansha): KITANI Shigeru 大阪大学, 生物工学国際交流センター, 准教授 (10379117) ; KINOSHITA Hiroshi 大阪大学, 生物工学国際交流センター, 助手 (20294035)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 放線菌 / 休眠生合成系 / 信号伝達物質 / 二次代謝

Outline of Final Research Achievements

Three kinds of Streptomyes autoregulators (virginiae butanolides, IM-2 and avenolide) were chemically synthesized and were added as a mixture to 50 representative Streptomyces species at the exponential phase at the concentration of 10 times the minimum effective concentration. By comparing the profiles of secondary metabolites with or without the addition of the autoregulator mixture, two strains (S. sahachiroi and S. narbonensis) were found to be influenced by the addition of the autoregulator mixture. To decide which of the three autoregulators is responsible for the change, each autoregulator was added alone, resulting to the finding that avenolide was responsible in both cases for the change of the secondary metabolites. These finding confirmed that the external addtion of Streptomyes autoregulators can be a tool to awaken the sleeping gene cluster for secondary metabolite in Streptomyces.

Free Research Field

分子微生物学