2012 Fiscal Year Final Research Report
Chemical Biology on the Symbiotic System in Marine Invertebrates
| Project/Area Number |
24651246
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Waseda University |
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Principal Investigator |
NAKAO Yoichi 早稲田大学, 理工学術院, 教授 (60282696)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Nobutada 独立行政法人産業技術総合研究所, プロセス研究部門, 主任研究員 (30344162)
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| Project Period (FY) |
2012
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| Keywords | 共生 / 海洋天然化合物 / 海綿 / 生合成 / シグナル / メタゲノム |
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| Research Abstract |
It has been disclosed that most of the marine secondary metabolites originate from the symbiotic microbes. In this research project, we focused on an unique marine metabolite, calyculin A from a marine sponge Discodermia calyx, and tried to separate and identify the calyculin A producing microbe. We have succeeded to obtain the fraction with concentrated microbes thought to produce calyculin A and extraction of genomic DNA from this assembly of the concentrated microbes. The metagenomic DNA sequencing analysis of the extracted genomic DNA and the following DNA mapping allowed us to identify the major microbe constituents and 13 contigs of PKS gene clusters.
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Research Products
(16 results)
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[Journal Article] Preliminary Structure-Activity Relationship on Theonellasterol, a New Chemotype of FXR Antagonist, from the Marine Sponge2012
Author(s)
Sepe, V. ; Ummarino, R. ; D'Auria, M. V. ; Taglialatela-Scafati, O. ; D'Amore, C. ; Renga, B. ; Nakao, Y. ; Fusetani, N. ; Fiorucci, S. ; Zampella, A. ; Marino, S. ; Chini, M. ; Bifulco, G.
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Journal Title
Theonella swinhoei Mar. Drugs
Volume: 10
Pages: 2448-2466
Peer Reviewed
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[Journal Article] Cristaxenicin A, an antiprotozoal xenicane diterpenoid from the deep sea gorgonian2012
Author(s)
Ishigami, S.-T. ; Goto, Y. ; Inoue, N. ; Kawazu, S.-I. ; Matsumoto, Y. ; Imahara, Y. ; Tarumi, M. ; Nakai, H. ; Fusetani, N. ; Nakao, Y.
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Journal Title
Acanthoprimnoa cristata J. Org. Chem.
Volume: 77
Pages: 10962-10966
Peer Reviewed
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