Comprehensive studies of genes responsible for biosynthesis of fusicoplagin diterpenoids in liverworts, and their use to development of reconstituted biosynthesis system

2013 Fiscal Year Final Research Report

• Project/Area Number: 24651247
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Tokushima Bunri University
• Principal Investigator: KENMOKU Hiromichi 徳島文理大学, 薬学部, 助教 (10399438)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 生合成 / 有用物質生産 / 分化誘導活性 / ジテルペノイド / フシコッカン / 次世代シーケンサー / Plagiochila sciophila / ケモタイプ

Research Abstract

Novel fusicoplagins, isolated from the leafy liverwort Plagiochila sciophila, are diterpenoids thay are potent inducers of differentiation in human myelocytic leukemia HL-60 cells. Because the fusicoplagins are a minuscule amount in the liverwort, construction of the stable supply method is a matter of great urgency. Therefore, studies aiming at the elucidation of the entire biosynthetic genes was carried out using a comprehensive transcriptome method. The predicted biosynthetic genes were discovered and the function of the diterpene cyclases was analyzed using heterologous gene expression system.

Research Products

• [Journal Article] Seed Dormancy Breaking Diterpenoids from the Liverwort Plagiochila sciophila and their Differentiation Inducing Activity in Human Acute Promyelocytic Leukemia HL-60 Cells (2014)
  • Author(s): Kenmoku, H., Tada, H., Oogushi, M., Esumi, T., Takahashi, T., Noji, M., Sassa, T., Toyota, M., Asakawa, Y.
  • Journal Title: Natural Product Communications Volume: 9(in printing)
  • Peer Reviewed
• [Journal Article] Seed Dormancy Breaking Diterpenoids, Including Novel Brassicicenes J and K, from Fungus Alternaria brassicicola, and their Necrotic/Apoptotic Activities in HL-60 Cells (2014)
  • Author(s): Kenmoku, H., Takeue, S., Oogushi, M., Yagi, Y., Sassa, T., Toyota, M., Asakawa, Y.
  • Journal Title: Natural Product Communications Volume: 9 Pages: 351-354
  • Peer Reviewed
• [Journal Article] Activity-guided isolation of cytotoxic bis-bibenzyl constituents from Dumortiera hirsuta (2013)
  • Author(s): Toyota, M., Ikeda, R., Kenmoku, H., Asakawa Y.
  • Journal Title: Journal of Oleo Science Volume: 62 Pages: 105-108
  • DOI: 10.5650/jos.62.105
  • Peer Reviewed
• [Presentation] ヤマブシタケ由来テルペン合成酵素によるジテルペン環化産物の構造解析 (2014)
  • Author(s): 兼目裕充,曽我達也,鴫原隆,木村榮一,佐々武史,高橋宏暢,野路征昭,豊田正夫,浅川義範
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市
  • Year and Date: 2014-03-30
• [Presentation] 糸状菌Alternaria brassicicolaからの14-3-3タンパク制御物質の探索 (2013)
  • Author(s): 兼目裕充,大櫛恵,竹上沙也加,佐々武史,豊田正夫,浅川義範
  • Organizer: 第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 松山市
  • Year and Date: 2013-10-27
• [Presentation] ヤマブシタケ由来テルペン合成酵素遺伝子の同定解析 (2013)
  • Author(s): 曽我達也,兼目裕充,鴫原隆,木村榮一,佐々武史,高橋宏暢,野路征昭,豊田正夫,浅川義範
  • Organizer: 第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 松山市
  • Year and Date: 2013-10-27
• [Presentation] ヤマブシタケ由来セスキテルペン合成酵素遺伝子群の網羅的同定解析 (2013)
  • Author(s): 曽我達也,兼目裕充,鴫原隆,木村榮一,佐々武史,高橋宏暢,野路征昭,豊田正夫,浅川義範
  • Organizer: 第57回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: さいたま市
  • Year and Date: 2013-10-07
• [Presentation] ヤマブシタケ由来エリナシン生合成酵素遺伝子群の網羅的同定解析 (2013)
  • Author(s): 曽我達也,兼目裕充,鴫原隆,木村榮一,佐々武史,高橋宏暢,野路征昭,豊田正夫,浅川義範
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-03-30
• [Presentation] 14-3-3タンパク制御物質の探索 (2013)
  • Author(s): 大櫛恵,兼目裕充,竹上沙也加,多田博幸,佐々武史,豊田正夫,浅川義範
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-03-30
• [Presentation] ゼニゴケ油体構成テルペノイドの生合成に関わる酵素遺伝子の同定と機能解析 (2012)
  • Author(s): 兼目裕充,森本樹奈,高橋宏暢,江角朋之,野路征昭,豊田正夫,三沢典彦,浅川義範
  • Organizer: 第56回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2012-10-29