2013 Fiscal Year Final Research Report
Methane synthesis using the budding yeast expression system
| Project/Area Number |
24651251
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
USUI Keiko 独立行政法人海洋研究開発機構, その他部局等, 研究員 (40608494)
TSUBOUCHI Taishi 独立行政法人海洋研究開発機構, その他部局等, 研究員 (30442990)
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| Project Period (FY) |
2012-04-01 – 2013-03-31
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| Keywords | メタン生合成 |
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| Research Abstract |
In this study, we be to integrate into the chromosome of S.cerevisiae the methane synthesis gene, it was intended to be the biosynthesis of methane in other archaea.
In methane biosynthesis, CoenzymeF420 synthesis-related genes expressed in S.cerevisiae. Result of studying protein is to be expressed and integrated into the transposon region of a chromosome of the host together with the promoter ADH1, it was not able to confirm expression. Future, we plan to in a way that gene replacement in a possible loss locus of S.cerevisiae, to continue the investigation or possible expression.
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