2014 Fiscal Year Final Research Report
Study on mitochondrial respiratory chain function using a hypoxia-sensing luminescent iridium complex probe
| Project/Area Number |
24651256
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOBITA Seiji 群馬大学, 大学院理工学府, 教授 (30164007)
TORII Seiji 群馬大学, 生体調節研究所, 准教授 (40312904)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | バイオテクノロジー / 生体機能利用 |
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| Outline of Final Research Achievements |
We have developed a red colour-emitting luminescent iridium complex probe BTP for detecting hypoxic region in vivo, and examined why reactive oxygen species ROS is more generated in a hypoxic condition than in a normoxic condition. We used primary-cultured rat cardiac muscle cells because their metabolic activity is easily controllable in a cellculture system. When the cells were stimulated by β-agonist, their beating movement increased in a dose-dependent manner, and they were turned hypoxic by BTO luminescent intensity. But this intensity was weakened with β-blocker.
Next, when we observed ROS generation with green colour-emitting fluorescent probe RT-TMROS, green colour was getting intensified with increase of BTP red color. But when active form of glutathione was added to the culture, green-colour was not augmented even in a hypoxic condition. We suggest that increased ROS generation in a hypoxic condition results from ROS-eliminating action by GSH-dependent peroxidase.
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| Free Research Field |
生体イメージング
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