2013 Fiscal Year Final Research Report
Development of new coelenterazine analogue for in vivo imaging of reactive oxygen species
| Project/Area Number |
24651257
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HOSOYA Takamitsu 東京医科歯科大学, 生体材料工学研究所, 教授 (60273124)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUYE Satoshi JNC株式会社, 横浜研究所, 主席研究員 東京工業大学・バイオフロンティアセンター・特任教授 (40426622)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 分子イメージング / 活性酸素種 / 生物発光 / ホウ素化合物 |
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| Research Abstract |
Reactive oxygen species are not only used as an intra- and intercellular messenger, but also involved in the onset of diseases such as inflammation or cancer. In this study, we aimed to develop an efficient method that can quantitatively detect reactive oxygen species in real time with high sensitivity. Since boron shows high affinity toward a certain type of reactive oxygen species, we developed a new synthetic method of organoboron-containing componds. We also synthesized a new coelenterazine analogue which served as an efficient substrate for a coelenterazine-type bioluminescence system.
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