2013 Fiscal Year Final Research Report
Signaling protein-imprinted polymer array having a fluorescent dye located within the imprinted cavity as a reporter molecule for the binding events
| Project/Area Number |
24651261
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 分子インプリンティング / 分子認識 / タンパク質 / 生体分子計測 |
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| Research Abstract |
A linker molecule comprised of a disulfide linkage to immobilize proteins for the preparation of signaling protein-imprinted polymers on substrates was designed and synthesized. The proteins coupled with the linker molecule maintained their secondary structure, and the molecular imprinting process properly proceeded. After the polymerization, the immobilized proteins were easily removed by the reduction of the disulfide moiety to leave the imprinted cavities capable of introducing thiol-reactive fluorescent dyes for signal transduction of binding events. Thickness of the protein imprinted polymer thin layer on the substrate was examined and, it is revealed that 10 to 20 nm thickness was suitable for the molecular recognition. Post-imprinting modification technique was developed to reduce the number of low affinity binding cavities, resulting in the improvement of S/N ratio.
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[Journal Article] Fluorescent Molecularly Imprinted Polymer Thin Films for Specific Protein Detection Prepared with Dansyl Ethylenediamine- Conjugated O-Acryloyl L-Hydroxyproline2013
Author(s)
Inoue, Y., Kuwahara, A., Ohmori, K., Sunayama, H., Ooya, T., Takeuchi, T
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Journal Title
Biosens. Bioelectron
Volume: 48
Pages: 113-119
DOI
Peer Reviewed
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