2014 Fiscal Year Final Research Report
Development of a science-educational program for experience of biological diversity and evolution at the DNA level using plant species
| Project/Area Number |
24653282
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Education on school subjects and activities
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| Research Institution | Kanagawa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 生物多様性 / 植物 / DNA / ポリメラーゼ連鎖反応法 / 制限酵素切断断片長分析 |
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| Outline of Final Research Achievements |
A comprehensive understanding of biodiversity is considered important when considering global environmental conservation. However, genetic diversity is less well known. We developed a simple practical program called ‘Kitchen PCR’ to communicate the concept of genetic diversity at the DNA level. Kitchen PCR essentially consists of three steps: Crude DNA extracts were prepared by homogenizing the plant materials in a Tris buffer, and the psbA-trnH intergenic region of the plastid genome was amplified by PCR. The amplified DNA fragments were fractionated by agarose gel electrophoresis and visualized by staining with ethidium bromide or Fast Blast DNA stain. Huge diversity the amplified DNA fragment lengths were clearly observed in the experimental plants. Kitchen PCR provides a simple method for demonstrating the concepts of genetic diversity at the DNA level and for introducing students to molecular biological techniques.
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| Free Research Field |
植物遺伝学、植物育種学、生物教育学
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