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2013 Fiscal Year Final Research Report

Study of effect of atmospheric pressure plasma on biological specimen by using in situ real time electron spin resonance technique

Research Project

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Project/Area Number 24654191
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plasma science
Research InstitutionNagoya University

Principal Investigator

ISHIKAWA Kenji  名古屋大学, 工学(系)研究科(研究院), 教授 (60417384)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsプラズマ滅菌 / 電子スピン共鳴 / フリーラジカル / ミドリカビ胞子
Research Abstract

Atmospheric pressure plasma sterilization of pathogens is now paid much attention for usefulness of no chemicals, electrical operations and so on. The plasma generates many kinds of reactive chemical species such as atomic oxygen, excited oxygen, hydroxyl radical, excited nitrogen, nitrous oxide, etc. However, interactions these species with biological specimens such as fungal spores, microorganisms etc. have not been clarified completely. In particular of intentional exposure of the reactive species to the biological specimen, the author has focused on understanding of free-radicals during plasma sterilization. Employing in situ real time electron spin resonance (ESR) technique, observation of free radical on the fungal spore of Penicillium digitatum has been succeeded. ESR signal decay is linked with inactivation of the spore, therefore the author found the close correlation of the free radical detected by ESR technique with biological response of inactivation on the fungal spores.

  • Research Products

    (8 results)

All 2014 2013 2012 Other

All Journal Article (3 results) Presentation (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Oxidation mechanism of Penicillium digitatum spores through neutral oxygen radicals2014

    • Author(s)
      Hiroshi Hashizume, Takayuki Ohta, Keigo Takeda, Kenji Ishikawa, Masaru Hori, Masafumi Ito
    • Journal Title

      Jpn. J. Appl. Phys

      Volume: 53 Pages: 010209

    • DOI

      10.7567/JJAP.53.010209

  • [Journal Article] Inactivation effects of neutral reactive-oxygen species on Penicillium digitatum spores using non-equilibrium atmospheric-pressure oxygen radical source2013

    • Author(s)
      Hiroshi Hashizume, Takayuki Ohta, Jia Fengdong, Keigo Takeda,Kenji Ishikawa,Masaru Hori, and Masafumi Ito
    • Journal Title

      Appl. Phys. Lett

      Volume: 103 (15) Pages: 153708

    • DOI

      10.1063/1.4824892

  • [Journal Article] Real-time In Situ Electron Spin Resonance Measurements on Fungal Spores of Penicillium digitatum during Exposure of Oxygen Plasmas2012

    • Author(s)
      Kenji Ishikawa, et al.
    • Journal Title

      Appl. Phys. Lett

      Volume: 101 (1) Pages: 013704

    • DOI

      10.1063/1.4733387

  • [Presentation] Electron spin resonance analyses of plasma-biological material interactions in atmospheric pressure plasmas2014

    • Author(s)
      Kenji Ishikawa, et al
    • Organizer
      International workshop on control of fluctuation of plasma processes - Joint International Workshop between "Frontier science of interactions between plasmas and nano-interfaces" and "Plasma medical innovation"
    • Place of Presentation
      福岡(3B-WS-07)
    • Year and Date
      20140200
  • [Presentation] In-situ ESR measurements for plasma materials interactions2013

    • Author(s)
      Kenji Ishikawa, et al
    • Organizer
      The 9th Asian-European International Conference on Plasma Surface Engineering (AEPSE)
    • Place of Presentation
      韓国(23-1)
    • Year and Date
      20130800
  • [Presentation] Electron spin resonance study of plasma-biological surface interaction for food hygiene2013

    • Author(s)
      Kenji Ishikawa, et al
    • Organizer
      The 5th International Symposium on Advanced Plasma Science and its applications for nitrides and nanomaterials (ISPlasma)
    • Place of Presentation
      名古屋(P3025A)
    • Year and Date
      20130300
  • [Remarks]

    • URL

      http://www.plasma.engg.nagoya-u.ac.jp/ishikawa/index.php/Home

  • [Patent(Industrial Property Rights)] 滅菌表示装置および滅菌装置および青果物表皮の殺菌方法2012

    • Inventor(s)
      石川健治、堀勝、伊藤昌文、太田貴之、橋爪博司
    • Industrial Property Rights Holder
      名古屋大学、名城大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      出願2012-037940
    • Filing Date
      2012-02-23

URL: 

Published: 2015-06-25  

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