2013 Fiscal Year Final Research Report
two-dimensional construction of ECM-glycoconjugate chips using single molecule manipulation by glycosyltransferase attached with AFM tip
| Project/Area Number |
24655098
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Polymer chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MORI Toshiaki 東京工業大学, 生命理工学研究科, 准教授 (50262308)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 1分子計測 / 糖転移反応 / AFM / フォースカーブ測定 / 糖鎖チップ |
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| Research Abstract |
In order to construct ECM-glycoconjugate chips, we employed the single molecule manipulation methods . First, we examine the preparation of AFM tips modified with glycosyltransferase. As a result, we could prepare the enzyme tips. And then, we performed the force-distance measurements with the enzyme tip to the substrates on the mica surface. We examined the interaction between enzymes and the corresponding substrates under several conditions. We confirm the detection of the procedures for the enzymatic reaction. And we could control the movoment of the tip in the area with 50 nm width.
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