2013 Fiscal Year Final Research Report
Construction and Functional Analysis of Artificial Thylakoid Membranes
| Project/Area Number |
24655120
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional materials chemistry
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
DEWA Takehisa 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (70335082)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 光合成膜 / 脂質二分子膜 / 人工チラコイド膜 / 積層膜 |
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| Research Abstract |
Thylakoid and photosynthetic bacterial membranes possess three-dimensional multi-layered structures. These membranes efficiently captured transfer light energy. In order to reveal the three-dimensional structure-function relationship, we constructed multi-layered planar membrane containing photosynthesis antenna complex (LH2). Bicelles containing biotinyl lipids were tethered to a planar lipid bilayer via avidin-biotin interaction. Upon detergent removal, transformation from bicelle to planar membrane took place to form layered planar bilayer. The layered structure was observed by atomic force microscopy (AFM). Multi-layered membranes containing LH2 complex was also constructed by use of LH2-reconstituted bicelles. Thus, we successfully constructed artificial thylakoid membranes.
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[Presentation] Molecular assembly of zinc chlorophyll derivatives by recombinant light-harvesting polypeptides with His-tag and immobilization on a gold electrode2013
Author(s)
S. Sakai, T. Noji, M. Kondo, T. Mizuno, T. Dewa, T. Ochiai, H. Yamakawa, S. Itoh, H. Hashimoto, M. Nango
Organizer
Light Harvesting Satellite Meeting 2013
Place of Presentation
Washington University, St. Louis
Year and Date
20130808-11
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