2013 Fiscal Year Final Research Report
Development of chromophore-assisted laser scarring method for characterization of drug target proteins
| Project/Area Number |
24655147
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOMATSU Toru 東京大学, 薬学研究科(研究院), 助教 (40599172)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 創薬化学 / ケミカルバイオロジー |
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| Research Abstract |
We have studied the detailed mechanisms of chromophore-assisted laser inactivation (CALI) to apply it for labeling drug target proteins in living cells for functional annotation. We have succeeded in confirming the usefulness of the method to selectively detect the small molecule-binding proteins on electrophoresis gels. In addition, we have established a novel method to characterize the proteins with desired enzymatic activities with use of small molecular fluorescent probes.
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[Journal Article]2013
Author(s)
Takahiro Egawa, Kazuhisa Hirabayashi, Yuichiro Koide, Chiaki Kobayashi, Naoya Takahashi, Tomoko Mineno, Takuya Terai, Tasuku Ueno, Toru Komatsu, Yuji Ikegaya, Norio Matsuki, Tetsuo Nagano and Kenjiro Hanaoka
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Journal Title
Angewandte Chemie International Edition
Volume: 52
Pages: 38740-3877
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[Journal Article] Development of a new class of azo-based fluorescence probes to detect different levels of hypoxia2013
Author(s)
Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, and Kenjiro Hanaoka
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Journal Title
Angewandte Chemie International Edition
Volume: 52
Pages: 13028-13032
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