Designing aggregated functional RNA medicine

2014 Fiscal Year Final Research Report

• Project/Area Number: 24655150
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Toyohashi University of Technology
• Principal Investigator: UMEKAGE SO 豊橋技術科学大学, 工学(系)研究科(研究院), 講師 (30419436)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: RNA / RNase / 凝集 / RNA医薬

Outline of Final Research Achievements

RNA is well known to be labile against nucleases attack, and it is assumed that using artificial ribonucleotides is inevitable for obtaining nuclease-resistance when preparing RNA therapeutics. In this research, we tried to obtain ribonuclease-resistant RNA transcripts by using only natural ribonucleotides. We found that in vitro transcripted RNA aggregated when meeting some specific conditions and these aggregates obtained both thermostable and ribonuclease-resistance. Also we showed that this aggregation depended on the length of DNA template and RNA contents. These findings will give significant insight into RNA engineering and/or RNA therapeutics when preparing functional RNAs.

Free Research Field

RNA医薬