2013 Fiscal Year Final Research Report
Development of site-specific conversion method of nucleobase in mRNA
| Project/Area Number |
24655152
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
HARI Yoshiyuki 大阪大学, 薬学研究科(研究院), 准教授 (50347423)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 核酸化学 |
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| Research Abstract |
In this study, we aimed at the development of the method for conversion ofcytosine nucleobase into uracil one in messenger RNA. In order to implement the method, sulnic acid-conjugated oligonucleotides were designed as a new class of modified oligonucleotides, and their syntheses were achieved. Although the conversion reaction of cytosine nucleobase within 2'-O-methyl-RNA using the synthesized oligonucleotides under various conditions was investigated during this research period, we could not succeed in the development of the conversion method. However, this reseach provided useful information for the development of the method in future.
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