2012 Fiscal Year Final Research Report
Regulation of secondary structure by RNA loop-loop interaction inducedsmall molecular binding
| Project/Area Number |
24655154
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012
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| Keywords | 生体認識 / 機能化学 / RNA / 小分子 |
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| Research Abstract |
In this study, we have challenged to modulate the secondary structure of RNA possessing two hairpin loops by small molecular binding. The molecule naphthyridine tetramer (NCT6) was found to bind two hairpin RNA possessing (CGG)3 sequence and make two RNA interacted. By using two hairpin RNA in one RNA construct, we have succeeded to induce secondary structural change by the binding of NCT6. The structural change was evidenced by observing FRET upon treating with NCT6.
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