Does the folate-dependent RNA methyltransferase regulate DNA replication?

2014 Fiscal Year Final Research Report

• Project/Area Number: 24655156
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Ehime University
• Principal Investigator: HORI Hiroyuki 愛媛大学, 理工学研究科, 教授 (20256960)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ＲＮＡ / RNA modification / methylation / DNA synthesis / folate / enzyme / thermophile

Outline of Final Research Achievements

The purpose of this sudy was the validation of our hypothesis, in which the folate-dependent RNA methyltransferase (TrmFO) regulates the DNA synthesis via consumption of 5, 10-methylenetetrahydrofolate. Under nutrient-poor condition, the trmFO gene disruptant strain of extreme-thermophilic eubacterium, Thermus thermophilus, grew faster than the wild-type strain. The slow growth of wild-type strain was accelarated by addition of thymine or thymidilate. These results supported the hypothesis. Furthermore, we determined the molecular ratio of TrmFO, 5, 10-methylenetetrahydrofolate synthase and thymidilate synthase. Our in vitro experiment revealed that TrmFO and thymidilate synthase compete each other via comsumption of 5, 10-methylenetetrahydrofolate.

Free Research Field

分子生物学