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2014 Fiscal Year Final Research Report

Does the folate-dependent RNA methyltransferase regulate DNA replication?

Research Project

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Project/Area Number 24655156
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemistry related to living body
Research InstitutionEhime University

Principal Investigator

HORI Hiroyuki  愛媛大学, 理工学研究科, 教授 (20256960)

Project Period (FY) 2012-04-01 – 2015-03-31
KeywordsRNA / RNA modification / methylation / DNA synthesis / folate / enzyme / thermophile
Outline of Final Research Achievements

The purpose of this sudy was the validation of our hypothesis, in which the folate-dependent RNA methyltransferase (TrmFO) regulates the DNA synthesis via consumption of 5, 10-methylenetetrahydrofolate. Under nutrient-poor condition, the trmFO gene disruptant strain of extreme-thermophilic eubacterium, Thermus thermophilus, grew faster than the wild-type strain. The slow growth of wild-type strain was accelarated by addition of thymine or thymidilate. These results supported the hypothesis. Furthermore, we determined the molecular ratio of TrmFO, 5, 10-methylenetetrahydrofolate synthase and thymidilate synthase. Our in vitro experiment revealed that TrmFO and thymidilate synthase compete each other via comsumption of 5, 10-methylenetetrahydrofolate.

Free Research Field

分子生物学

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Published: 2016-06-03  

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