Development of biorelated-material-inspired ionic liquid for a visualizing agent for electron microscopic observations

2013 Fiscal Year Final Research Report

• Project/Area Number: 24656026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Hokkaido University
• Principal Investigator: YONEZAWA TETSU 北海道大学, 工学(系)研究科(研究院), 教授 (90284538)
• Co-Investigator(Kenkyū-buntansha): ABE Shigeaki 北海道大学, 大学院歯学研究科, 助教 (40374566)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 電子顕微鏡 / 走査型電子顕微鏡 / イオン液体 / 可視化 / 導電性 / 生体試料 / 赤血球 / コリン

Research Abstract

In this study, we have used ionic liquids as the SEM visualizing reagent of non-conductive nanomaterials and bio-related samples including cells and construct an optimized pre-treatment method for SEM observation. Ionic liquids are organic salts which are in liquid at room temperature and do not evaporate even in a vacuum conditions. Therefore, they should be a suitable material for SEM visualization.
We have been able to establish the processes to visualize non-conductive nanomaterials during this research period. For bio-related samples, including cells, we applied choline-type structured ionic liquids as the visualizing agents. The samples were dispersed in the ionic liquids and put on a membrane filter. The dispersion was filtered gently under reduced pressure by using a syringe and excess ionic liquids were removed. Using this process, red blood cells could be observed by SEM without any deformation.

Research Products

• [Journal Article] Influence of surface properties for SEM observation of carbon nanotubes prepared with room temperature ionic liquid (2014)
  • Author(s): Shigeaki Abe, Atsushi Hyono, and Tetsu Yonezawa
  • Journal Title: Journal of Solution Chemistry Volume: Vol. 43(印刷中)
  • Peer Reviewed
• [Journal Article] Static and time lapse observation for estimation of biocompatibility of nano-sized ceramic particles with osteoblasts and hepatocytes (2014)
  • Author(s): Shigeaki Abe, Nobuki Iwadera, Yusuke Hamba, Atsushi Hyono, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, Jun-ichiro Iida, and Tetsu Yonezawa
  • Journal Title: Biomatter Volume: Vol. 4(印刷中)
  • Peer Reviewed
• [Journal Article] Visualizing preparation using asymmetrical choline-like ionic liquids for scanning electron microscope observation of non-conductive biological samples (2014)
  • Author(s): Shigeaki Abe, Atsushi Hyono, Koji Kawai, and Tetsu Yonezawa
  • Journal Title: Journal of Nanoscience and Nanotechnology Volume: Vol. 14 Pages: 2620-2623
  • Peer Reviewed
• [Journal Article] SEM observation of the live morphology of human red blood cells under high vacuum conditions using a novel RTIL (2014)
  • Author(s): Atsushi Hyono, Tetsu Yonezawa, Koji Kawai, Shigeaki Abe, Mitsuhiro Fujihara, Hiroshi Azuma, and Shinobu Wakamoto
  • Journal Title: Surface and Interface Analysis Volume: Vol. 46(印刷中) Pages: 425-428
  • Peer Reviewed
• [Journal Article] Direct SEM observation of non-electroconductive TiO_2 nanotube arrays prepared by anodization using an ionic liquid as a visualizaing reagent (2013)
  • Author(s): Chaorui Xue, Takashi Narushima, and Tetsu Yonezawa
  • Journal Title: Journal of Inorganic & Organic Polymers & Materials Volume: Vol. 23(印刷中) Pages: 239-242
  • Peer Reviewed
• [Journal Article] Simple preatreatment of non-conductive small hydrous bio-samples with choline-type ionic liquid and membrane filter for microsample mounting (2013)
  • Author(s): Koji Kawai, Kotaro Kaneko, Hayato Kawakami, Takashi Narushima, Tetsu Yonezawa
  • Journal Title: Colloids and Surfaces B–Biointerfaces Volume: Vol 102 Pages: 9-12
  • Peer Reviewed
• [Journal Article] SEM observation of nano carbon materials with imidazolium-type room temperature ionic liquid (2013)
  • Author(s): Shigeaki Abe, Atsushi Hyono, Katsutoshi Nakayama, Tomoya Takada, and Tetsu Yonezawa
  • Journal Title: Japanese Journal of Applied Physics Volume: Vol 52 Pages: 01AH02
• [Journal Article] Conducitivity preparation of choline lactate ethanol solution for SEM observation (2012)
  • Author(s): Shigeaki Abe, Atsushi Hyono, Yuri Machida, Fumio Watari, and Tetsu Yonezawa
  • Journal Title: Both hard and soft tissues in living matter Volume: Vol 4 Pages: 18-23
• [Presentation] イオン液体における応用技術 (2014)
  • Author(s): 米澤徹
  • Organizer: ScanTech 2014
  • Place of Presentation: 東京都市大学世田谷キャンパス, 東京都
  • Year and Date: 2014-09-12
  • Invited
• [Presentation] イオン液体を用いたバイオマテリアルのSEM新規観察法の開発 (2014)
  • Author(s): 兵野篤, 阿部薫明, 赤坂司, 亘理文夫, 米澤徹
  • Organizer: 代用臓器・再生医学研究会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-03-01
• [Presentation] Biocompatibility and biodistribution of several micro-/nano-sized inorganic particles (2013)
  • Author(s): Shigeaki Abe, Atsushi Hyono, Yusaku Hamba, Nobuki Iwadera, Shuuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, J.-i. Iida, and Tetsu Yonezawa
  • Organizer: 6th International Symposium on Nanotechnology, Occupational and Environmental Health (NanOEH)
  • Place of Presentation: Nagoya Congress Center, 愛知県
  • Year and Date: 2013-10-29
• [Presentation] Scanning electron microscope observation of nano/biological materials with room temperature ionic liquids (2013)
  • Author(s): Shigeaki Abe, Atsushi Hyono, Koji Kawai, and Tetsu Yonezawa
  • Organizer: International Conference on Solution Chemistry
  • Place of Presentation: Kyoto Tersa, 京都府
  • Year and Date: 2013-07-10
• [Presentation] イオン液体を用いたナノ/バイオマテリアルのSEM観察 (2013)
  • Author(s): 阿部薫明, 兵野篤, 河合功治, 米澤徹
  • Organizer: 医学生物学顕微鏡技術学会
  • Place of Presentation: 神奈川歯科大学, 神奈川県
  • Year and Date: 2013-06-08
• [Presentation] イオン液体による肝細胞由来スフェロイドのSEM観察 (2013)
  • Author(s): 許斐麻美, 塩野正道, 坂上万里, 中澤英子, 河合功治, 桑畑進, 米澤徹
  • Organizer: 日本顕微鏡学会第69学術講演会
  • Place of Presentation: ホテル阪急液スポパーク, 大阪府
  • Year and Date: 2013-05-20
• [Presentation] イオン液体を用いた柔組織のSEM観察 (2013)
  • Author(s): 坂上万里, 許斐麻美, 塩野正道, 中澤英子, 河合功治, 桑畑進, 米澤徹
  • Organizer: 日本顕微鏡学会第69学術講演会
  • Place of Presentation: ホテル阪急液スポパーク, 大阪府
  • Year and Date: 2013-05-20
• [Presentation] SEM観察における生体試料可視化のためのイオン液体の利用 (2013)
  • Author(s): 阿部薫明, 兵野篤, 河合功治, 亘理文夫, 米澤徹
  • Organizer: 日本顕微鏡学会第69学術講演会
  • Place of Presentation: ホテル阪急液スポパーク, 大阪府
  • Year and Date: 2013-05-20
• [Presentation] イオン液体の電子顕微鏡応用 (2012)
  • Author(s): 中澤英子, 許斐麻美, 塩野正道, 河合功治, 桑畑進, 米澤徹
  • Organizer: 日本顕微鏡学会第56回シンポジウム
  • Place of Presentation: 北海道大学, 北海道
  • Year and Date: 20121119-20
  • Invited
• [Presentation] コリン型イオン液体による含水物質への導電性付与とSEM観察への応用 (2012)
  • Author(s): 米澤徹
  • Organizer: 日本顕微鏡学会第56回シンポジウム
  • Place of Presentation: 北海道大学, 北海道
  • Year and Date: 20121119-20
• [Presentation] チタニアナノチューブアレーの簡便なSEM観察法 (2012)
  • Author(s): 薜超瑞, 成島隆, 米澤徹
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば国際会議場, 茨城県
  • Year and Date: 20120514-16
• [Presentation] イオン液体を用いたナノカーボン物質の微細構造の観察 (2012)
  • Author(s): 阿部薫明, 兵野篤, 亘理文夫, 米澤徹
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば国際会議場, 茨城県
  • Year and Date: 20120514-16
• [Presentation] Double-walled TiO_2 nanotube arrays prepared by anodization in fluoride/glycerol electrolyte (2012)
  • Author(s): Chaorui Xue, Takashi Narushima, and Tetsu Yonezawa
  • Organizer: 2012 International Joint Symposium between HU & CNU on Advanced Material Science and Technology
  • Place of Presentation: 北海道大学, 北海道
  • Year and Date: 2012-11-30
• [Remarks] 先進材料ハイブリッド工学研究室ホームページ
  • URL: http://labs.eng.hokudai.ac.jp/labo/limsa/