2013 Fiscal Year Final Research Report
Development of biorelated-material-inspired ionic liquid for a visualizing agent for electron microscopic observations
Project/Area Number |
24656026
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Thin film/Surface and interfacial physical properties
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Research Institution | Hokkaido University |
Principal Investigator |
YONEZAWA TETSU 北海道大学, 工学(系)研究科(研究院), 教授 (90284538)
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Co-Investigator(Kenkyū-buntansha) |
ABE Shigeaki 北海道大学, 大学院歯学研究科, 助教 (40374566)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Keywords | 電子顕微鏡 / 走査型電子顕微鏡 / イオン液体 / 可視化 / 導電性 / 生体試料 / 赤血球 / コリン |
Research Abstract |
In this study, we have used ionic liquids as the SEM visualizing reagent of non-conductive nanomaterials and bio-related samples including cells and construct an optimized pre-treatment method for SEM observation. Ionic liquids are organic salts which are in liquid at room temperature and do not evaporate even in a vacuum conditions. Therefore, they should be a suitable material for SEM visualization. We have been able to establish the processes to visualize non-conductive nanomaterials during this research period. For bio-related samples, including cells, we applied choline-type structured ionic liquids as the visualizing agents. The samples were dispersed in the ionic liquids and put on a membrane filter. The dispersion was filtered gently under reduced pressure by using a syringe and excess ionic liquids were removed. Using this process, red blood cells could be observed by SEM without any deformation.
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[Presentation] Biocompatibility and biodistribution of several micro-/nano-sized inorganic particles2013
Author(s)
Shigeaki Abe, Atsushi Hyono, Yusaku Hamba, Nobuki Iwadera, Shuuichi Yamagata, Fumio Watari, Tsukasa Akasaka, Yasutaka Yawaka, J.-i. Iida, and Tetsu Yonezawa
Organizer
6th International Symposium on Nanotechnology, Occupational and Environmental Health (NanOEH)
Place of Presentation
Nagoya Congress Center, 愛知県
Year and Date
2013-10-29
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[Presentation] イオン液体の電子顕微鏡応用2012
Author(s)
中澤英子, 許斐麻美, 塩野正道, 河合功治, 桑畑進, 米澤徹
Organizer
日本顕微鏡学会第56回シンポジウム
Place of Presentation
北海道大学, 北海道
Year and Date
20121119-20
Invited
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