2012 Fiscal Year Final Research Report
Challenging Exploratory Study of Odor Recorder Using Olfactory Receptors
| Project/Area Number |
24656253
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Measurement engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANZAKI Ryohei 東京大学, 先端科学技術研究センター, 教授 (40221907)
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| Project Period (FY) |
2012
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| Keywords | センシングデバイス |
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| Research Abstract |
The fundamental study of an odor recorder using olfactory receptors of an insect was performed. Especially, sensor devices are important since they influence the performance of the odor recorder very much. We used the olfactory receptors of the insect since their mechanism is much simpler than that of a vertebrate. In this study, we established the cell systems with the stable expressions of five olfactory receptors by transferring drosophila olfactory-receptor gene into Sf21 cell together with the gene of fluorescent protein sensitive to calcium, followed by the antibiotic screening. The optical imaging reveals that the odor concentration can be measured using fluorescent response of each cell system to odorant. Thus, it was found that the established cell system can be used as a sensor component.Then, we studied the simple fluorescent measurement system. We fabricated the measurement cell, where a power LED illuminated the cells and their fluorescence was detected by a photo de via an optical filter. Although we performed the experiments on fluorescent beads and the cells, it was found that the light intensity from the power LED was insufficient. However, the large fluorescent intensity was obtained when we used a laser and improved the illumination method. Moreover, we investigated the feasibility of odor approximation by blending several odor components by simulation when we used the responses of multiple olfactory receptors to odorants accumulated in the database.
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