2013 Fiscal Year Final Research Report
Development of a rapid and simple method based on fluorescence polarization for quantification of waterborne viruses
| Project/Area Number |
24656307
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 水系感染症 / ノロウイルス / 蛍光偏光法 / カプシドタンパク質 / 抗原抗体反応 / 金コロイド / 3DNA / 複合体 |
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| Research Abstract |
The objective of this study was to develop a rapid and simple method applying fluorescence polarization which has been used as a tool to evaluate protein-protein interaction for quantification of waterborne viruses. As a result, novel method that can detect norovirus particles within 30 min was successfully developed based on the investigation of effects of plate type, antibody type, antibody concentration and antigen-antibody reaction time on virus detection by fluorescence polarization.
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