2013 Fiscal Year Final Research Report
Circadian rhythmic control of differentiation of human stem cells
| Project/Area Number |
24656495
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAGI Mutsumi 北海道大学, 工学(系)研究科(研究院), 教授 (20263212)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Masashi 北海道大学, 大学院工学研究院, 助教 (30229075)
HONMA Ken-ichi 北海道大学, 大学院医学研究科, 特任教授 (40113625)
NISHIDE Shinya 北海道大学, 大学院医学研究科, 助教 (40451398)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞培養 / 時計遺伝子 / 間葉系幹細胞 / 分化 / トランスジェニックラット / グルコース / デキサメタゾン / 血清 |
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| Research Abstract |
The effects of various cultivation operation on re-oscillation of circadian gene expression , especially on amplitude and phase of oscillation, were quantitatively clarified. The phase at the beginning of re-oscillation depended on the kind of cultivation operation including medium change, additions of glucose, large amount of serum, and dexamethasone, which knowledge might contribute on not only the study about the mechanism of re-oscillation but also the control methodology of the oscillation of circadian gene expression.
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