2013 Fiscal Year Final Research Report
Development of selective cell collection method using photo-degradable PEG-lipid
| Project/Area Number |
24656498
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAMUNE Teruyuki 東京大学, 工学(系)研究科(研究院), 教授 (20124373)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Satoshi 東京大学, 先端科学技術研究センター, 講師 (80398106)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 光分解性PEG脂質 / 細胞パターニング / 一細胞アレイ / 細胞分離回収 |
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| Research Abstract |
Single-cell array is a promising technology for microscopy-based high-content analyses.In this study, we constructed a versatile single cell array for both non-adherent and adherent cells by developing a photo-cleavable PEG-lipid/collagen coating material. On this material surface, we confirmed that cells behave similar to their native functions such as cell adhesion, mitosis and migration. Furthermore, we succeeded in high-throughput single cell quantitative imaging analysis on the motility of the adherent cells and that on the morphological changes of non-adherent cells using this PEG-lipid/collagen-based single-cell array, and the recovery of a target cell by photo-irradiation of this cell.This versatile single-cell array shall be invaluable tool for the research in the fields of cell engineering.
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Research Products
(12 results)
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[Journal Article] Transfer Pronting of Transfected Cell Microarrays from Poly(ethylene glycol)-oleyl Substrates onto Biological Hydrogels2013
Author(s)
Yamaguchi, S., Komiya, S., Matsunuma, E., Yamahira, S., Kihara, Y., Miyake, J. and Nagamune, T.
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Journal Title
Biotechnol. Bioeng.
Volume: 110
Pages: 3269-3274
DOI
Peer Reviewed
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