Chiral lipidomics by using supercritical fluid chromatography/mass spectrometry

2014 Fiscal Year Final Research Report

• Project/Area Number: 24656503
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: BAMBA Takeshi 大阪大学, 工学(系)研究科(研究院), 教授 (10432444)
• Co-Investigator(Renkei-kenkyūsha): FUKUSAKI Eiichiro 大阪大学, 大学院工学研究科, 教授 (40273594) ; YOSHIDA Masaru 神戸大学, 大学院医学研究科, 准教授 (00419475)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: enantiomer / lipidomics / supercritical fluid / mass spectrometry / chiral analysis

Outline of Final Research Achievements

We applied supercritical fluid chromatography (SFC) to the enantiomeric separation of lipids and tried to construct the chiral lipidomics system. We successfully separated triacylglycerol (TAG) enantiomeric isomers with connected chiral columns. Additionally, SFC also allows us to separate TAG positional isomers. Furthermore, we performed the analyses of biologicalsamples by using the constructed chiral lipidomics system. We achieved the enantiomeric separation of TAG enantiomeric isomers and positional isomers in edible oils. Additionally, we performed the chiral lipidomics in diabetes and myocardial infarction samples and obtained specific profiles.

Free Research Field

metabolomics, supercritical fluid engineering