2012 Fiscal Year Final Research Report
Analysis and application of new translation system
| Project/Area Number |
24656511
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ABE Hiroshi 独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (80415067)
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| Project Period (FY) |
2012
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| Keywords | mRNA / 翻訳 / タンパク質合成 / 環状RNA / 原核生物 / リボソーム / 終わりのない回転式翻訳 |
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| Research Abstract |
Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis.
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