Investigation of Daphnia as a genetic model organism

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657022
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ecology/Environment
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: IGUCHI Taisen 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡﨑統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ミジンコ / 逆遺伝学 / エコゲノミクス / 甲殻類

Research Abstract

The ubiquitous, freshwater microcrustacean Daphnia has been analyzed as a model animal for monitoring ecosystem integrity. Daphnia are capable of either clonal or sexual reproduction, making them ideally suited for genetic research, but the establishment of in vitro development of the embryos and gene modification techniques has not been established. We investigate a condition for natural male production and found the Daphnia strain whose sex is solely regulated by day length. In addition, we established an optimal condition for microinjections and the following in vitro development of the embryos. The current research is preliminary but our findings provide useful knowledge for further study for genetic study for Daphnids.

Research Products

• [Journal Article] Establishment of a short-term, in vivo screening method for detecting chemicals with juvenile hormone activity using adult Daphnia magna (2014)
  • Author(s): Abe R, Watanabe H, Yamamuro M, Iguchi T, Tatarazako N
  • Journal Title: J Appl Toxicol Volume: (in press)
  • DOI: 10.1002/jat.2989
  • Peer Reviewed
• [Journal Article] Development of a microinjection system for RNA interference in the water flea Daphnia pulex (2014)
  • Author(s): Hiruta C, Toyota K, Miyakawa H, Ogino Y, Miyagawa S, Tatarazako N, Shaw JR, Iguchi T
  • Journal Title: BMC Biotechnol Volume: 13 Pages: 96
  • DOI: 10.1186/1472-6750-13-96
  • Peer Reviewed
• [Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna (2014)
  • Author(s): Toyota K, Kato Y, Miyakawa H, Yatsu R, Mizutani T, Ogino Y, Miyagawa S, Watanabe H, Nishide H, Uchiyama I, Tatarazako N, Iguchi T
  • Journal Title: J Appl Toxicol Volume: 34 Pages: 537-544
  • DOI: 10.1002/jat.2922
  • Peer Reviewed
• [Journal Article] A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans (2013)
  • Author(s): Miyakawa H, Toyota K, Hirakawa I, Ogino Y, Miyagawa S, Oda S, Tatarazako N, Miura T, Colbourne JK, Iguchi T
  • Journal Title: Nat Commun Volume: 4 Pages: 1856
  • DOI: 10.1038/ncomms2868
  • Peer Reviewed
• [Journal Article] Genomic expression responses toward bisphenol-A toxicity in Daphnia magna in terms of reproductive activity (2013)
  • Author(s): Jeong SW, Lee SM, Yum SS, Iguchi T, Seo YR
  • Journal Title: Mol. Cell. Toxicol Volume: 9 Pages: 149-158
  • URL: http://link.springer.com/article/10.1007/s13273-013-0019-y
  • Peer Reviewed
• [Journal Article] Science and policy on endocrine disrupters must not be mixed : a reply to a"common sense"intervention by toxicology journal editors (2013)
  • Author(s): Bergman Å, Andersson AM, Becher G, van den Berg M, Blumberg B, Bjerregaard P, Bornehag CG, Bornman R, Brandt I, Brian JV, Casey SC, Fowler PA, Frouin H, Giudice LC, Iguchi T, Hass U, Jobling S, Juu A, Kidd KA, Kortenkamp A, Lind M, Martin OV, Muir D, Ochieng R, Olea N, Norrgren L, Ropstad E, Ross PS, Rudén C, Scheringer M, Skakkebaek NE, Söder O, Sonnenschein C, Soto A, Swan S, Toppari J, Tyler CR, Vandenberg LN, Vinggaard AM, Wiberg K., Zoeller RT.
  • Journal Title: Environ. Health Volume: 12 Pages: 69
  • DOI: 10.1186/1476-069X-12-69
  • Peer Reviewed
• [Journal Article] The impact of endocrine disruption : A consensus statement on the state of the science (2013)
  • Author(s): Bergman Å, Heindel JJ, Kidd KA, Jobling S, Zoeller RT, Becher G, Bjerregaard P, Bornman R, Brandt I, Brian JV, Kortenkamp A, Muir D, Ochieng R, Skakkebaek NE, Iguchi T, Toppari J
  • Journal Title: Environ. Health Perspect. Volume: 121 Pages: A104-106
  • DOI: 10.1289/ehp.1205448
  • Peer Reviewed
• [Journal Article] Molecular cloning of doublesex genes of four cladocera (water flea) species (2013)
  • Author(s): Toyota K, Kato Y, Sato M, Sugiura N, Miyagawa S, Miyakawa H, Watanabe H, Oda S, Ogino Y, Hiruta C, Mizutani T, Tatarazako N, Paland S, Jackson C, Colbourne JK, Iguchi T
  • Journal Title: BMC Genomics Volume: 14 Pages: 239
  • DOI: 10.1186/1471-2164-14-239
  • Peer Reviewed
• [Presentation] Approach to establishing tools for gene functional analysis and cryopreservation in Daphnia pulex (2014)
  • Author(s): Hiruta C, Toyota K, Miyakawa H, Miyagawa S, Ogino Y, Tatarazako N, Shaw JR, Tanaka D, Iguchi T
  • Organizer: Daphnia Genomics Consortium 2014
  • Place of Presentation: University of Birmingham, イギリス
  • Year and Date: 20140120-22
• [Presentation] Abortive meiosis found in the oogenesis of parthenogenetic Daphnia pulex (2014)
  • Author(s): Hiruta C, Tochinai S, Iguchi T
  • Organizer: Daphnia Genomics Consortium 2014 meeting
  • Place of Presentation: University of Birmingham, イギリス
  • Year and Date: 20140120-22
• [Presentation] A single amino acid substitution in Methoprene-tolerant(Met) alters juvenile hormone use by insects and Crustaceans (2013)
  • Author(s): Miyakawa H, Toyota K, Hirakawa I, Ogino Y, Miyagawa S, Oda S, Tatarazako N, Miura T, Colbourne JK, Iguchi T
  • Organizer: Insect Hormones International Workshop 2013
  • Place of Presentation: University of Minnesota,アメリカ
  • Year and Date: 20130721-26
• [Presentation] Environmental sex determination of water flea, Daphnia magna (2013)
  • Author(s): Iguchi T, Toyota K., Miyakawa H, Hiruta C, Ogino Y, Miyagawa S, Tatarazako N
  • Organizer: 17th International Congress of Comparative Endocrinology (ICCE 2013)
  • Place of Presentation: Facultat de Biologia at the Universitat de Barcelona, スペイン
  • Year and Date: 20130715-19
• [Presentation] Development of an RNA interference method using microinjection in the water flea Daphnia pulex (2013)
  • Author(s): Hiruta C, Toyota K, Miyakawa H, Iguchi T
  • Organizer: 46th Ann. Meet. Jap. Soc. Devel. Biol.
  • Place of Presentation: 島根県松江市
  • Year and Date: 20130528-31
• [Presentation] Aquatic environments and endocrine activity : The regulatory approach in Japan (2013)
  • Author(s): Iguchi T
  • Organizer: SETAC Europe 23rd Ann. Meet.
  • Place of Presentation: Glasgow, イギリス
  • Year and Date: 20130512-16
• [Remarks]
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html