Live imaging of unusual behaviors of tapetal cells in Arabidopsis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657030
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: ISHIGURO Sumie 名古屋大学, 生命農学研究科, 准教授 (50260039)
• Co-Investigator(Renkei-kenkyūsha): HIGASHIYAMA Tetsuya 名古屋大学, 大学院理学研究科, 教授 (00313205)
• Research Collaborator: SUZUKI Toshiya 名古屋大学, 大学院生命農学研究科, 研究員
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物 / 生殖 / 花粉 / 有糸分裂 / タペート細胞 / イメージング

Outline of Final Research Achievements

Tapetal cells are nursery cells for developing pollen grains in anthers. One of the most characteristic features of the cells is binucleation. Live imaging revealed that the binucleation proceeds synchronously in tapetal tissue. During the process, we found that spindles formed normally but phragmoplast disappeared without producing cell plates. In the late stage of anther development, we observed tapetal cells become protoplasts and migrate interstitially through pollen grains in locules. Then they collapse and release their components to the surroundings of pollen grains. It may contribute to form uniform pollen coats on the surface of all pollen grains in locules.

Free Research Field

植物分子遺伝学