Toward compilation of an atlas of Marchantia polymorpha, a model basal land plants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657031
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: ARAKI Takashi 京都大学, 生命科学研究科, 教授 (00273433)
• Research Collaborator: YAMAGUCHI Ayako ; ENDO Motomu ; SAKAI Yuuki ; MIWA Masaki ; HIGO Asuka ; MORIHANA Sayuri ; OGAMI Takashi ; YAMAMOTO Akito
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ゼニゴケ / 発生 / 生殖器官 / 精子形成 / 精子機能 / 胞子体 / 組織特異的遺伝子 / エンハンサートラップ

Outline of Final Research Achievements

Through RNA seq analysis of isolated antheridia, a comprehensive list of genes specifically expressed during antheridial development was obtained, which enables us to draw a framework for gene expression program of the process. The list contains genes expressed in spermatogenous tissue at specific stage of development, which will serve as useful markers. A gene likely to be involved in early stages of sexual organ development was also identified through analysis of knock-out plants of selected transcription factor genes. These will be useful resources for us to contribute to our understanding sexual organ development. Other outputs from this project of a notable value include establishment of lines which mark apical notch region of thallus and catalogues of genes expressed in archegonia before and after fertilization.
In association to this project, I am serving as one of editors for the specially focused issue on Marchantia polymorpha as a model plant in Plant & Cell Physiology.

Free Research Field

植物生理・分子生物学