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2013 Fiscal Year Final Research Report

Studies on chloroplast personalities by visualizing cellular signal transduction processes and photosynthesis.

Research Project

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Project/Area Number 24657036
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto Prefectural University

Principal Investigator

SHIINA Takashi  京都府立大学, 生命環境科学研究科(系), 教授 (10206039)

Co-Investigator(Kenkyū-buntansha) KUMAZAKI Shigeichi  京都大学, 大学院理学研究科, 准教授 (40293401)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords葉緑体 / Ca2+ / ROS / 表皮 / 免疫 / ストロミュール
Research Abstract

Different tissues contain various types of plastids that differ in function and morphology. This study revealed that plastids have distinct functions in plant immunity and Ca2+ signaling in a tissue-specific manner. Plastids in leaves and roots evoked distinct Ca2+ signals in response to biotic and abiotic stresses such as salt and osmotic stresses. Furthermore, we found that plastids in epidermis have a specific role in biosynthesis of salicylic acid in plant immunity. Interestingly, we demonstrated that Ca2+ and ROS signaling play critical roles in the induction of stromules in chloroplasts in epidermal cells of hypocotyles. These results imply that plastids in distinct tissues have specialized roles.

  • Research Products

    (24 results)

All 2014 2013 Other

All Journal Article (6 results) Presentation (18 results)

  • [Journal Article] Recent advances in the study of chloroplast gene expression and its evolution2014

    • Author(s)
      Yagi, Y and Shiina, T.
    • Journal Title

      Front. in Plant Sci

      Volume: (eCollection 2014) Pages: 5-61

  • [Journal Article] Calcium signaling in plant endosymbiotic organelles : mechanism and role in physiology2014

    • Author(s)
      Nomura, H. and Shiina, T.
    • Journal Title

      Mol. Plant

      Volume: (in press)

  • [Journal Article] A scalable open-source pipeline for large-scale root phenotyping2014

    • Author(s)
      Radka Slovak, Christian Göschl, Xiaoxue Su, Koji Shimotani, Takashi Shiina and Wolfgang Busch
    • Journal Title

      Plant Cell

      Volume: (in press)

  • [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana2013

    • Author(s)
      Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
    • Journal Title

      Plant Signal Behav

      Volume: 8 Pages: e23603

  • [Journal Article] Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering2013

    • Author(s)
      Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 77 Pages: 2140-3

  • [Journal Article] Transformation of thylakoid membranes during differentiation from vegetative cell into heterocyst visualized by microscopic spectral imaging

    • Author(s)
      Kumazaki S, Akari M, Hasegawa M.
    • Journal Title

      Plant Physiol

      Volume: 161 Pages: 1321-1333

  • [Presentation] AMPが誘導する病害抵抗瀬遺伝子発現と光合成電子伝達2014

    • Author(s)
      青山真夕、中井香奈、下谷紘司、佐野智、椎名隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Year and Date
      20140318-20
  • [Presentation] 葉緑体Ca^<2+>センサーCASを介した防御応答遺伝子発現の制御2014

    • Author(s)
      下谷 紘司、青山 真夕、中井 香奈、佐野智、椎名 隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Year and Date
      20140318-20
  • [Presentation] ミトコンドリア局在機械受容チャネルMSL1の機能解析2014

    • Author(s)
      艾原佐紀,原田尚実,市川美恵,古市卓也、椎名隆
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山
    • Year and Date
      20140318-20
  • [Presentation] A role for chloroplast Ca^<2+> sensor protein CAS in regulation of intracellular Ca2+ signaling and plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      Indo-Japan Joint Workshop on "Signal sensing and transduction in photosynthetic organisms-from cyanobacteria to land plants"
    • Place of Presentation
      ハイデラバード インド
    • Year and Date
      20131216-18
  • [Presentation] Role of chloroplasts in plant defense responses2013

    • Author(s)
      Shiina, T.
    • Organizer
      International Closing Meeting of the Research Unit 804‚ Retrograde Signaling in Plants
    • Place of Presentation
      ミュンヘン ドイツ
    • Year and Date
      20131013-16
  • [Presentation] ミトコンドリア機械受容チャネルMSL1の機能解析2013

    • Author(s)
      艾原佐紀,原田尚実,市川美恵,椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      20130913-15
  • [Presentation] 葉緑体accD 遺伝子プロモータの進化2013

    • Author(s)
      石崎陽子,木寅翔太,新村周一,椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      20130913-15
  • [Presentation] 葉緑体Ca^<2+>センサーCASによる植物の免疫応答制御の解析2013

    • Author(s)
      下谷紘司,中井香奈,山崎加奈子,佐野智、椎名隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      20130913-15
  • [Presentation] 葉緑体ストロミュールのABAによる誘導機構2013

    • Author(s)
      椎名隆、金麗花
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌
    • Year and Date
      20130913-15
  • [Presentation] Functional Characterization of Mitochondrial Mechanosensitive Channel MSL1 in Arabidopsis thaliana2013

    • Author(s)
      Yomogihara, S., Harada, N., Asakura, C., Yamaguchi, S., Ichikawa, M., Furuichi, T., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Year and Date
      20130624-28
  • [Presentation] Control of defense gene expression via chloroplast Ca^<2+> sensor protein CAS in Arabidopsis2013

    • Author(s)
      Shimotani1, K., Nakai1, K., Nomura, H., Yoshioka, H., Shiina, T.
    • Organizer
      第24回国際シロイヌナズナ研究会議
    • Place of Presentation
      シドニー オーストラリア
    • Year and Date
      20130624-28
  • [Presentation] Chloroplast Ca^<2+> and plant immunity2013

    • Author(s)
      Shiina, T.
    • Organizer
      3rd International Symposium on Chloroplast Genomics and Genetic
    • Place of Presentation
      ニューブルンスイック 米国
    • Year and Date
      20130508-11
  • [Presentation] 葉緑体光転写制御におけるコムギ葉緑体RNAポリメラーゼ複合体のDNA結合動態の解析2013

    • Author(s)
      本村幸也,八木祐介,中平洋一,戸澤譲,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300
  • [Presentation] ウルシノキのトランスクリプトーム解析2013

    • Author(s)
      石崎陽子,岡本嘉明,佐藤貴彦,大藪泰,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300
  • [Presentation] ミトコンドリアCa^<2+> センサータンパク質AtMICU1の機能解析2013

    • Author(s)
      山口咲希,八木祐介,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300
  • [Presentation] チラコイド膜CASタンパク質が制御する防御関連転写因子群の解析2013

    • Author(s)
      下谷紘司,神田ゆい,中平洋一,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300
  • [Presentation] 光条件に依存する植物病理応答遺伝子の発現解析2013

    • Author(s)
      中井香奈,金麗花,中平洋一,椎名隆
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300
  • [Presentation] 植物免疫における葉緑体ラジカル制御タンパク質CASの下流で働く因子の単離と解析2013

    • Author(s)
      野村裕也,藤原正幸,深尾陽一朗,椎名隆,吉岡博文
    • Organizer
      第53回日本植物生理学会
    • Place of Presentation
      京都
    • Year and Date
      20130300

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Published: 2015-06-25  

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