2015 Fiscal Year Final Research Report
Cell culture method for the study of secretory activation in mammary epithelial cells
| Project/Area Number |
24657044
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Tsukuba |
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Principal Investigator |
|
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| Research Collaborator |
KASAI Hiroaki
INOUE Naoya
NEMOTO Tasuku
OKAMURA Naomichi
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| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Keywords | lactation / tissue culture / milkstasis / histamine |
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| Outline of Final Research Achievements |
Mammary epithetial cells produce and secrete milk in response to a full set of appropriate systemic and local stimuli. Lack of one of the necessary signals attenuates full activation of lactation. Here, it has been revealed that local signals such as histamine/H1 receptor, in addition to systemic hormone such as insulin and cortisol, contribute to milkstasis via maintaining polarity of the mammary epithelial cells. Although all of these signals were found to be necessary for milk secretion in vivo, the best cocktail of these factors were not yet sufficient to make mammary epithetial cells produce abundant milk in vitro. Innovation of bioreactor to produce milk awaits further studies.
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| Free Research Field |
mammary biology
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