Novel approaches to insect learning systems using transgenic crickets

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hokkaido University
• Principal Investigator: MIZUNAMI Makoto 北海道大学, 理学(系)研究科(研究院), 教授 (30174030)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: コオロギ / 学習 / 遺伝子組換え / RNA干渉 / 記憶 / 遺伝子発現阻害 / ドーパミン / オクトパミン

Research Abstract

We attempted to establish (1) transgenic techniques and (2) RNA interference (RNAi) methods to study molecular basis of learning in crickets, Gryllus bimaculatus. For the former, we successfully obtained heterozygous knockout individuals of type 1 dopamine receptor (DopR1) by using CRISPR/CAS system, and now we are now trying to obtain homozygous DopR1 knockout crickets by inbreeding them. For the latter, we performed RNAi experiments with to types of dopamine receptors (Dop1 and Dop2) and a type of octopamine receptor (OA1) and obtained evidence to show that DopR1 participates in aversive learning and OA1 participates in appetitive condoning, whereas DA2 participates in neither of them. The results provided convincing evidence that octopamine neurons and dopamine neurons mediate reward and punishment signals, respectively, in associative learning in crickets.

Research Products

• [Journal Article] Cyclic nucleotide–gated channels, calmodulin, adenylyl cyclase and calcium/calmodulin-dependent kinase II are required for late but not early long-term memory formation in the honey bee (2014)
  • Author(s): Matsumoto Y, Sandoz JC, Devaud JM, Lormant F, Mizunami M, Giurfa M
  • Journal Title: Learning & Memory Volume: 21 Pages: 272-286
  • DOI: 10.1101/lm.032037.113
  • Peer Reviewed
• [Journal Article] Roles of NO signaling in long-term memory formation in visual learning in an insect (2013)
  • Author(s): Matsumoto Y, Hirashima D, Terao K, Mizunami M
  • Journal Title: PLoS One Volume: 8 Pages: e68538
  • DOI: 10.1371/journal.pone.0068538
  • Peer Reviewed
• [Journal Article] Analysis and modeling of neural processes underlying sensory preconditioning (2013)
  • Author(s): Matsumoto Y, Hirashima D, Mizunami M
  • Journal Title: Neurobiol. Learn. Mem Volume: 101 Pages: 103-113
  • DOI: 10.1016/j.nlm.2013.01.008
  • Peer Reviewed
• [Journal Article] Participation of NO signaling in formation of long-term memory in salivary conditioning of the cockroach (2013)
  • Author(s): Matsumoto C.S., Kuramochi T, Matsumoto Y, Watanabe H, Nishino H, Mizunami M
  • Journal Title: Neurosci. Lett. Volume: 541 Pages: 4-8
  • DOI: 10.1016/j.neulet.2013.01.010
  • Peer Reviewed
• [Journal Article] Divergent and convergent projections to the two parallel olfactory centers from two neighboring, pheromone-receptive glomeruli in the male American cockroach (2012)
  • Author(s): Nishino H, Iwasaki M, Kamimura I, Mizunami M
  • Journal Title: J. Comp. Neurol. Volume: 520 Pages: 3428-3445
  • DOI: 10.1002/cne.23111
  • Peer Reviewed
• [Journal Article] Visual and olfactory input segregation in the mushroom body calyces in a basal neopteran, the American cockroach (2012)
  • Author(s): Nishino H., Iwasaki M., Yasuyama K., Hongo H., Watanabe H., Mizunami M.
  • Journal Title: Arthropod Struct. Dev. Volume: 41 Pages: 3-16
  • DOI: 10.1016/j.asd.2011.08.005
  • Peer Reviewed
• [Journal Article] Context-dependent olfactory learning monitored by activities of salivary neurons in cockroaches (2012)
  • Author(s): Matsumoto C.S., Matsumoto Y., Watanabe H., Nishino H., and Mizunami M.
  • Journal Title: Neurobiol. Learn. Mem Volume: 97 Pages: 30-36
  • DOI: 10.1016/j.nlm.2011.08.010
  • Peer Reviewed
• [Presentation] Intelligence with microbrains : Cognitive processes underlying insect learning (2014)
  • Author(s): Mizunami M
  • Organizer: International workshop on animal instinctive and intelligent behaviors
  • Place of Presentation: Hokkaido Univ. (Sapporo)
  • Year and Date: 2014-02-20
  • Invited
• [Presentation] コオロギの古典的条件づけにおけるブロッキング (2013)
  • Author(s): 寺尾勘太,松本幸久、水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-09-26
• [Presentation] コオロギ古典的条件付けにおける習慣形成とオクトパミンニューロンの関係の解明 (2013)
  • Author(s): 廣鰭翔,松本幸久,水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山市
  • Year and Date: 2013-09-26
• [Presentation] RNA干渉法によるコオロギの学習・記憶の分子メカニズムの解析 (2013)
  • Author(s): 和久田亮,中村太郎,松本幸久,野地澄晴,三戸太郎,水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-09-26
• [Presentation] フタホシコオロギの加齢性記憶障害における飼育環境の影響 (2013)
  • Author(s): 松本幸久,松本-佐藤千尋,中野真樹,服部淳彦,水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-09-26
• [Presentation] MERを用いたゴキブリの学習 (2013)
  • Author(s): 細野翔平,松本幸久,水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-09-26
• [Presentation] コオロギにおけるブロッキング現象の確認 (2013)
  • Author(s): 寺尾勘太,松本幸久,水波誠
  • Organizer: 日本動物学会北海道支部会
  • Place of Presentation: 北海道教育大学(札幌市)
  • Year and Date: 2013-08-24
• [Presentation] ゴキブリを用いた新しい学習実験系の確立 (2013)
  • Author(s): 細野翔平,松本幸久,水波誠
  • Organizer: 日本動物学会北海道支部会
  • Place of Presentation: 北海道教育大学(札幌市)
  • Year and Date: 2013-08-24
• [Presentation] RNAiによるコオロギの記憶形成に関わる分子機構の解明 (2013)
  • Author(s): 和久田亮,松本幸久,水波誠
  • Organizer: 日本動物学会北海道支部会
  • Place of Presentation: 北海道教育大学(札幌市)
  • Year and Date: 2013-08-24
• [Presentation] Analysis and modeling of sensory preconditioning in crickets (2013)
  • Author(s): Mizunami M, Matsumoto Y, Hirashima D
  • Organizer: 日本比較生理生化学会
  • Place of Presentation: イーグレひめじ(姫路市)
  • Year and Date: 2013-07-13
• [Presentation] Memory molecules in insects : from behavioral pharmacological studies of olfactory learning in crickets. Symposium : Potential of insect neuroscience (2012)
  • Author(s): Matsumoto Y, Matsumoto C, Mizunami M
  • Organizer: Japanese Society for Neuroscience, 日本神経科学学会
  • Place of Presentation: 名古屋コンベンションセンター(名古屋市)
  • Year and Date: 2012-09-20
  • Invited
• [Presentation] コオロギ古典的条件付けにおける習慣形成の行動学的証明 (2012)
  • Author(s): 廣鰭翔、松本幸久、水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪大学(大阪市)
  • Year and Date: 2012-09-15
• [Presentation] フタホシコオロギの嗅覚学習におけるPKCシグナルの役割解析 (2012)
  • Author(s): 川中子沙織、松本幸久、水波誠
  • Organizer: 日本動物学会
  • Place of Presentation: 大阪大学(大阪市)
  • Year and Date: 2012-09-15
• [Presentation] コオロギ古典的条件付けにおける習慣形成 (2012)
  • Author(s): 廣鰭翔、松本幸久、水波誠
  • Organizer: 日本動物学会北海道支部会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-08-25
• [Presentation] Analysis and modeling of sensory preconditioning in crickets (2012)
  • Author(s): Mizunami M, Matsumoto Y, Hirashima D
  • Organizer: 10th International Congress of Neuroethology, Maryland
  • Place of Presentation: Univ. Maryland (USA)
  • Year and Date: 2012-07-19
• [Presentation] Roles of aminergic neurons in memory acquisition and retrieval in crickets (2012)
  • Author(s): Mizunami M
  • Organizer: International Symposium : Biogenic Amines in Insects
  • Place of Presentation: Berlin Free University (Germany)
  • Year and Date: 2012-07-07
  • Invited
• [Presentation] Olfactory conditioning of mandible elevation response in cricket (2012)
  • Author(s): Matsumoto Y, Matsumoto C, Mizunami M
  • Organizer: 日本比較生理生化学会
  • Place of Presentation: 総合研究大学院大学(葉山市)
  • Year and Date: 2012-07-07
• [Presentation] NO-cGMP signaling is involved in age-related memory impairment in the cricket Gryllus bimaculatus (2012)
  • Author(s): Matsumoto Y, Matsumoto C, Mizunami M
  • Organizer: Second International Conference on the Cricket
  • Place of Presentation: Tokushima Univ. (Tokushima)
  • Year and Date: 2012-03-21
  • Invited
• [Presentation] Systemic RNA interference for the study of long-term memory formation in the cricket (2012)
  • Author(s): Mizunami M, Takahashi T, Hamada A, Miyawaki K, Matsumoto Y, Mito T, Noji S
  • Organizer: Second International Conference on the Cricket
  • Place of Presentation: Tokushima Univ. (Tokushima)
  • Year and Date: 2012-03-19
  • Invited
• [Book] 行動生物学辞典(上田恵介他編、自己受容器などの項目や付録1を担当) (2013)
  • Author(s): 水波誠
  • Publisher: 東京化学同人
• [Book] 微小脳と巨大脳(項目担当)、高校生物教師用指導書(DVD版) (2013)
  • Author(s): 水波誠
  • Publisher: 東京書籍
• [Book] Chapter 41: Olfactory and visual learning in cockroaches and crickets. In : Invertebrate Learning and Memory, eds by R. Menzel and P.R.Benjamin (2013)
  • Author(s): Mizunami M., Matsumoto Y., Watanabe H., Nishino H.
  • Total Pages: 547-558
  • Publisher: Springer
• [Book] マダガスカルゴキブリの飼育(分担執筆)、針山孝彦他編、研究者が教える動物飼育第2巻「昆虫とクモの仲間」 (2012)
  • Author(s): 松本幸久、水波誠
  • Total Pages: 31-34
  • Publisher: 共立出版
• [Remarks] ①朝日新聞グローブ 「脳の不思議、鳥や虫の脳もすごい!」 2014年3月16日(取材協力) 報道関係情報
• [Remarks] ②朝日新聞 「トンボの目玉はどうなっている?」 2012年6月9日(取材協力) 報道関係情報
• [Remarks] ①北海道大学オープンキャンパス 高校生限定プログラム「昆虫の学習を体験しよう!」 2013年8月5日 北海道大学(札幌市) アウトリーチ活動
• [Remarks] 日本生物教育会全国大会実験研修「"脳"をみんなの教材に」講師 2012年8月4日 北海道大学(札幌市) アウトリーチ活動
• [Remarks]
  • URL: https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/