Developmental studies of a new bio-profiling method for linking morphology and genomic information of eukaryotic microorganisms.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24657060
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: University of Tsukuba
• Principal Investigator: YOKOYAMA Akiko 筑波大学, 生命環境系, 研究員 (30466601)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Takeshi 筑波大学, 生命環境系, 講師 (40302369)
• Project Period (FY): 2012-04-01 – 2013-03-31
• Keywords: 藻類 / プロティスト

Research Abstract

Metagenomic studies using environment sequences reveals that huge numbers of unknown species, including completely new phylogenetic groups, existed in the eukaryotic microorganisms. On the other hand, some of taxonomically described species might be recognized as unknown organisms because their sequences data have not been accumulated. In this study, we performed trial investigations to make a comprehensive and efficient bio-profiling method for unknown, hardly culturable, or high speed swimming eukaryotic microorganisms.

Research Products

• [Journal Article] 13^2,17^3-Cyclopheophorbide b enol as a catabolite of chlorophyll b in phycophagy by protists (2013)
  • Author(s): Kashiyama Y., Yokoyama A., Shiratori T., Inouye I., Kinoshita Y., Mizoguchi T. and Tamiaki H.
  • Journal Title: FEBS Letters Volume: 587 Pages: 2578-2583
  • DOI: 10.1016/j.febslet.2013.06.036
  • Peer Reviewed
• [Journal Article] 藻類食プロティストが関与するクロロフィルの「解毒」代謝 (2013)
  • Author(s): 横山亜紀子・柏山祐一郎
  • Journal Title: 藻類 Volume: 61(1) Pages: 6-10
• [Journal Article] Ubiquity and quantitative significance of detoxification catabolism of chlorophyll associated with protistan herbivory (2012)
  • Author(s): Kashiyama Y., Yokoyama A., Kinoshita Y., Shoji S., Miyashiya H., Shiratori T., Suga H., Ishikawa K., Ishikawa A., Inouye I., Ishida K., Fujinuma D., Aoki K., Kobayashi M., Nomoto S., Mizoguchi T. and Tamiaki H.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109(43) Pages: 1728-1735
  • DOI: 10.1073/pnas.1207347109
  • Peer Reviewed
• [Presentation] 藻類・プロティストの代謝分解と共生進化との関連 (2014)
  • Author(s): 柏山祐一郎,横山亜紀子,白鳥峻志,中澤昌美,鈴木利幸,土屋正史,鏡味麻衣子,柴田あいか,早川昌志,川原純,梅谷貴大,民秋均
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 東邦大学(船橋)
  • Year and Date: 2014-03-16
• [Presentation] 真核生物のかたちとゲノムをつなぐ〜高速度撮影による形態観察の有用性と問題点〜 (2014)
  • Author(s): 横山亜紀子
  • Organizer: 第1回コンタ研究会
  • Place of Presentation: 筑波大学下田臨海実験センター(下田)
  • Year and Date: 2014-01-30
• [Presentation] 光合成内部共生進化に関わったユーグレノイドのクロロフィル解毒代謝 (2013)
  • Author(s): 柏山祐一郎,横山亜紀子,民秋均
  • Organizer: 第2回マトリョーシカ型生物学研究会
  • Place of Presentation: ホテル京都ガーデンパレス(京都)
  • Year and Date: 2013-07-25
• [Presentation] 光合成内部共生進化に関わったユーグレノイドのクロロフィル解毒代謝 (2013)
  • Author(s): 柏山祐一郎,横山亜紀子,民秋均
  • Organizer: 日本地球惑星連合2013年大会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-05-19
• [Presentation] 沿岸域におけるクロロフィル誘導体と真核微生物相の時空間変動解析 (2013)
  • Author(s): 横山亜紀子,柏山祐一郎,守屋繁春,民秋均,井上勲
  • Organizer: 日本地球惑星連合2013年大会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2013-05-19
• [Presentation] プロティスト捕食活動の指標としてのシクロエノールの可能性を探る (2013)
  • Author(s): 横山亜紀子,柏山祐一郎,守屋繁春,民秋均,井上勲
  • Organizer: 日本藻類学会第37回大会
  • Place of Presentation: 山梨大学(甲府)
  • Year and Date: 2013-03-28
• [Presentation] 色素体獲得を可能にしたユーグレノイドによるクロロフィルの解毒代謝分解 (2013)
  • Author(s): 柏山祐一郎,横山亜紀子,白鳥峻志,川口明音,中沢昌美,井上勲,民秋均
  • Organizer: 日本藻類学会第37回大会
  • Place of Presentation: 山梨大学(甲府)
  • Year and Date: 2013-03-28
• [Presentation] Chlorophyll catabolism in aquatic ecosystems : Physiology, ecology, and evolution (2013)
  • Author(s): Kashiyama Y., Yokoyama A. and Tamiaki H.
  • Organizer: CER International workshop on Biogeochemical cycling and Microbial Ecology for Young Scientists
  • Place of Presentation: 京都大学生態学研究センター(大津)
  • Year and Date: 2013-03-19
• [Presentation] Detoxification catabolism of chlorophyll associated with protistan herbivory in aquatic ecosystems (2012)
  • Author(s): Kashiyama Y., Yokoyama A. and Tamiaki H.
  • Place of Presentation: 立命館大学(草津)
  • Year and Date: 2012-12-01
• [Presentation] Seasonal succession of eukaryotic community detected by environmental sequences in Tokyo Bay, Japan (2012)
  • Author(s): Yokoyama A., Kikuchi J., Tsuboi Y., Moriya S., Inagaki Y., Hashimoto T. and Inouye I.
  • Organizer: Protist 2012
  • Place of Presentation: Oslo,Norway
  • Year and Date: 2012-08-02
• [Presentation] Morphology and phylogeny of a new imbricatean flagellate (phylum Cercozoa) (2012)
  • Author(s): Shiratori T., Yokoyama A. and Ishida K.
  • Organizer: Protist 2012
  • Place of Presentation: Oslo,Norway
  • Year and Date: 2012-08-02
• [Presentation] Ecological significance of chlorophyll metabolism of herbivorous protists in aquatic ecosystems (2012)
  • Author(s): Kashiyama Y., Yokoyama A., Miyashita H., Ishikawa K., Ishikawa A., Inouye I. and Tamiaki H.
  • Organizer: Protist 2012
  • Place of Presentation: Oslo,Norway
  • Year and Date: 2012-08-01
• [Presentation] Evolutionary significance of chlorophyll metabolisms of herbivorous protists in aquatic ecosystems (2012)
  • Author(s): Kashiyama Y., Yokoyama A., Shiratori T., Inouye I., Ishida K. and Tamiaki H.
  • Organizer: Protist 2012
  • Place of Presentation: Oslo,Norway
  • Year and Date: 2012-07-30
• [Presentation] 微細藻類捕食プロティストによるクロロフィルの解毒代謝 (2012)
  • Author(s): 柏山祐一郎,横山亜紀子,木下雄介,庄司淳,宮下英明,白鳥峻志,菅寿美,石川可奈子,石川輝,井上勲,石田健一郎,藤沼大幹,青木啓介7・小林正美,野本信也,溝口正,民秋均
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-07-15
• [Presentation] 塩基配列情報で探る東京湾沿岸の真核微生物相とその季節変動 (2012)
  • Author(s): 横山亜紀子,守屋繁春,稲垣祐司,橋本哲男,井上勲
  • Organizer: 日本藻類学会第36回大会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-07-14
• [Presentation] Occurrence and distributiono of Chlorophyll catabolites in aquatic environments (2012)
  • Author(s): Kashiyama, Y., Yokoyama, A., Kinoshita, Y., Miyashita, H., Ishikawa, K., Ishikawa, A, Mizoguchi, T., Tamiaki, H.
  • Organizer: 2012 ASLO. Aquatic Sciences meeting, Voyages of Discovery
  • Place of Presentation: 滋賀県立びわ湖ホール(大津)
  • Year and Date: 2012-07-12